CAS Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
ACS Appl Bio Mater. 2021 Jan 18;4(1):406-419. doi: 10.1021/acsabm.0c00353. Epub 2020 Dec 23.
The success of complex tissue and internal organ reconstruction relies principally on the fabrication of a 3D vascular network, which guarantees the delivery of oxygen and nutrients in addition to the disposal of waste. In this study, a rapidly forming cell-encapsulated double network (DN) hydrogel is constructed by an ultrasonically activated silk fibroin network and bioorthogonal-mediated polyethylene glycol network. This DN hydrogel can be solidified within 10 s, and its mechanical property gradually increases to ∼20 kPa after 30 min. This work also demonstrates that coencapsulation of human umbilical vein endothelial cells (HUVECs) and umbilical cord-derived mesenchymal stem cells (UCMSCs) into the DN hydrogel can facilitate the formation of more mature vessels and complete the capillary network in comparison with the hydrogels encapsulated with a single cell type both and . Taking together, the DN hydrogel, combined with coencapsulation of HUVECs and UCMSCs, represents a strategy for the construction of a functional vascular network.
复杂组织和内部器官重建的成功主要依赖于 3D 血管网络的构建，该网络可保证除废物处理外，氧气和营养物质的输送。在本研究中，通过超声激活丝素蛋白网络和生物正交介导的聚乙二醇网络构建了一种快速形成的细胞包封的双网络（DN）水凝胶。该 DN 水凝胶可在 10s 内固化，其力学性能在 30min 后逐渐增加到约 20kPa。这项工作还表明，与人脐静脉内皮细胞（HUVECs）和脐带间充质干细胞（UCMSCs）共包封到 DN 水凝胶中可促进更成熟血管的形成，并与单独包封单一细胞类型的水凝胶相比，完成毛细血管网络的形成。综上所述，DN 水凝胶与 HUVECs 和 UCMSCs 的共包封代表了构建功能性血管网络的一种策略。
