Shandong Engineering Research Center for Smart Materials and Regenerative Medicine, College of Pharmacy, Weifang Medical University, Weifang 261053, P. R. China.
Department of Chemistry, Korea University, Seoul 02841, Korea.
ACS Appl Bio Mater. 2021 Mar 15;4(3):2080-2088. doi: 10.1021/acsabm.0c01178. Epub 2020 Nov 6.
Peroxynitrite (ONOO), a powerful biological oxidant, is produced in the mitochondria and reacts with many biomolecular targets under various pathological conditions, leading to a range of disease states. In this work, we developed a nanoliposome-encapsulated ratiometrically fluorescent probe () based on a hemicyanine structure obtained by facile synthesis. Upon reaction with ONOO, the oxidation and hydrolysis of a π-conjugation system within the nanoliposome triggers a ratiometrically fluorescent response and a large-scale emission shift (238 nm), which provides a specific and sensitive means for the ONOO detection. Moreover, we have performed DFT calculation at the 6-31+G(d,p) level using a suite of programs to obtain insights into the chemical structure optical properties of . In addition, the practical applications of the nanoprobe to image exogenous and endogenous ONOO were achieved further in live cells and animals triumphantly.
过氧亚硝酸盐（ONOO-）是一种强生物氧化剂，在各种病理条件下产生于线粒体，并与许多生物分子靶标发生反应，导致一系列疾病状态。在这项工作中，我们通过简便的合成方法得到了一种基于半花菁结构的纳米脂质体包裹的比率荧光探针（）。与 ONOO-反应后，纳米脂质体内部的π共轭体系的氧化和水解会引发比率荧光响应和大规模的发射位移（238nm），为 ONOO-的检测提供了一种特异而灵敏的手段。此外，我们还使用了一系列程序在 6-31+G(d,p)水平上进行了密度泛函理论（DFT）计算，以深入了解的化学结构和光学性质。此外，该纳米探针在外源性和内源性 ONOO-的成像中的实际应用也在活细胞和动物中取得了成功。
