Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC.
Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC.
ACS Appl Bio Mater. 2021 Mar 15;4(3):2591-2600. doi: 10.1021/acsabm.0c01577. Epub 2021 Jan 28.
Nitric oxide (NO) gas nanocarrier materials were prepared via a hierarchical assembly of poly(amido amine) dendrimers with fluorocarbon binding sites (DEN-) and fluorinated poly(ethylene glycol) (-PEG) on nitrogen-doped carbon nanohorns (NCNHs). The loading abilities of NO gas in these nanocarrier materials increased with the nitrogen doping of CNH and hierarchies formed by DEN- and -PEG. Especially, the ability of CNH-based nanocomposite materials was better than that of graphene-based materials. The loading of NO gas arose an infrared absorption band at 1387 cm and increased the intensity ratio of D and G bands in Raman spectra, although these phenomena diminished after the degas treatment. The antimicrobial effects on bacteria ( and ) increased depending on the loading amount of NO gas. It was confirmed from these results that NO gas weakly interacts with nitrogen-doped CNH and is trapped in the void volumes of DEN- and -PEG hierarchies. Thus, the concentric hierarchy is preferable for slow release of NO gas due to the void volumes in DEN-, -PEG, and CNH hierarchical organization. This sustained release of NO gas is advantageous with regards to the potential biomedical gas therapy against bacteria and other parasites.
通过在氮掺杂碳纳米角（NCNH）上组装具有氟碳结合位点（DEN-）和氟化聚乙二醇（-PEG）的聚酰胺胺树枝状大分子（DEN-）和-PEG，制备了一氧化氮（NO）气体纳米载体材料。这些纳米载体材料中 NO 气体的负载能力随 CNH 的氮掺杂和 DEN-和-PEG 形成的分层而增加。特别是，基于 CNH 的纳米复合材料的能力优于基于石墨烯的材料。NO 气体的负载引起了在 1387cm 处的红外吸收带，并且增加了拉曼光谱中 D 和 G 带的强度比，尽管这些现象在脱气处理后减少。对细菌（和）的抗菌作用取决于 NO 气体的负载量。从这些结果可以确认，NO 气体与氮掺杂的 CNH 弱相互作用，并被捕获在 DEN-和-PEG 分层的空隙体积中。因此，由于 DEN-、-PEG 和 CNH 分层组织中的空隙体积，同心分层有利于 NO 气体的缓慢释放。这种 NO 气体的持续释放对于针对细菌和其他寄生虫的潜在生物医学气体治疗是有利的。
