Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei 106, Taiwan, ROC.
ACS Appl Bio Mater. 2021 Apr 19;4(4):3203-3213. doi: 10.1021/acsabm.0c01600. Epub 2021 Mar 18.
Herein, a hierarchical structure of flower-like NiCo layered double hydroxides (NiCo LDH) microspheres composed of three-dimensional (3D) ultrathin nanosheets was successfully synthesized via a facile hydrothermal approach. The formation of NiCo LDH was confirmed by various physicochemical studies, and the NiCo LDH-modified glassy carbon electrode was used as an efficient dual-functional electrocatalyst for non-enzymatic glucose and hydrogen peroxide (HO) biosensor. The host matrix of hydrotalcite NiCo LDH exhibits the enhanced electrocatalytic sensing performances with a quick response time (<3 s), wide linear range (50 nM-18.95 mM and 20 nM-11.5 mM) and lowest detection limits (S/N = 3) (10.6 and 4.4 nM) toward glucose and HO, and also it exhibits good stability, selectivity, and reproducibility. In addition, this biosensor was successfully utilized to the real-time detection of endogenous HO produced from live cells and glucose in various biological fluids, and demonstrates that the as synthesized NiCo LDH may provide a successful pathway for physiological and clinical pathological diagnosis.
在此，通过简便的水热法成功合成了由三维（3D）超薄纳米片组成的花状 NiCo 层状双氢氧化物（NiCo LDH）微球的分层结构。通过各种物理化学研究证实了 NiCo LDH 的形成，并且将 NiCo LDH 修饰的玻碳电极用作非酶葡萄糖和过氧化氢（HO）生物传感器的有效双功能电催化剂。水滑石 NiCo LDH 的主体基质表现出增强的电催化传感性能，具有快速的响应时间（<3 s），宽线性范围（50 nM-18.95 mM 和 20 nM-11.5 mM）和最低检测限（S/N = 3）（10.6 和 4.4 nM）对葡萄糖和 HO 的检测，并且还表现出良好的稳定性，选择性和重现性。此外，该生物传感器还成功地用于实时检测来自活细胞和各种生物流体中的内源性 HO 和葡萄糖的检测，并证明所合成的 NiCo LDH 可能为生理和临床病理诊断提供了成功的途径。
