Flexible nickel-cobalt double hydroxides micro-nano arrays for cellular secreted hydrogen peroxide in-situ electrochemical detection.

It is critical to detect cellular secreted hydrogen peroxide (HO) in situ for clinical diagnosis, biomedical research and cancer treatment. Herein, the electrochemical determination of HO released by cancer cells grown on the surface of carbon cloth supported NiCo-DH/AuPt micro-nano arrays to elevate the capability of in situ signal collection was achieved. NiCo-DH/AuPt @CC was successfully prepared using the cobalt based metal-organic framework (Co-MOF) as a presoma after in situ etching growth onto the CC and electrodeposition of gold and platinum nanoparticles (AuPt NPs). Under the optimal conditions, owing to the excellent catalytic efficiency of NiCo-DH and AuPt NPs, the designed sensor performs a relatively wider linear range to HO concentration from 10 μM to 22.08 mM, and the limit of detection is 0.145 μM. Accordingly, the as-prepared sensing system was also applied to determine HO secreted by living cells which grown on the surface of NiCo-DH/AuPt @CC with satisfactory consequences. In possession of the superior sensitivity, selectivity, reproducibility, the NiCo-DH/AuPt @CC is a luciferous platform for the real time detection of HO in the area of biomedical and clinical diagnosis.