Zhao Jiaying, Yang Huisi, Wu Weixuan, Shui Zhengfan, Dong Jiangbo, Wen Li, Wang Xueqi, Yang Mei, Hou Changjun, Huo Danqun
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
Anal Chim Acta. 2021 Jan 25;1143:135-143. doi: 10.1016/j.aca.2020.11.047. Epub 2020 Nov 30.
It is critical to detect cellular secreted hydrogen peroxide (HO) in situ for clinical diagnosis, biomedical research and cancer treatment. Herein, the electrochemical determination of HO released by cancer cells grown on the surface of carbon cloth supported NiCo-DH/AuPt micro-nano arrays to elevate the capability of in situ signal collection was achieved. NiCo-DH/AuPt @CC was successfully prepared using the cobalt based metal-organic framework (Co-MOF) as a presoma after in situ etching growth onto the CC and electrodeposition of gold and platinum nanoparticles (AuPt NPs). Under the optimal conditions, owing to the excellent catalytic efficiency of NiCo-DH and AuPt NPs, the designed sensor performs a relatively wider linear range to HO concentration from 10 μM to 22.08 mM, and the limit of detection is 0.145 μM. Accordingly, the as-prepared sensing system was also applied to determine HO secreted by living cells which grown on the surface of NiCo-DH/AuPt @CC with satisfactory consequences. In possession of the superior sensitivity, selectivity, reproducibility, the NiCo-DH/AuPt @CC is a luciferous platform for the real time detection of HO in the area of biomedical and clinical diagnosis.
对于临床诊断、生物医学研究和癌症治疗而言，原位检测细胞分泌的过氧化氢（HO）至关重要。在此，实现了对生长在碳布负载的NiCo-DH/AuPt微纳阵列表面的癌细胞释放的HO进行电化学测定，以提高原位信号收集能力。在碳布上原位蚀刻生长并电沉积金和铂纳米颗粒（AuPt NPs）后，以钴基金属有机框架（Co-MOF）为前驱体成功制备了NiCo-DH/AuPt@CC。在最佳条件下，由于NiCo-DH和AuPt NPs具有优异的催化效率，所设计的传感器对HO浓度在10μM至22.08 mM范围内具有相对较宽的线性范围，检测限为0.145μM。因此，所制备的传感系统还用于测定生长在NiCo-DH/AuPt@CC表面的活细胞分泌的HO，结果令人满意。凭借卓越的灵敏度、选择性和重现性，NiCo-DH/AuPt@CC是生物医学和临床诊断领域中用于实时检测HO的一个有前景的平台。
