College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou 362000, Fujian, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
ACS Appl Bio Mater. 2021 Feb 15;4(2):1424-1431. doi: 10.1021/acsabm.0c01314. Epub 2021 Jan 21.
Ractopamine as an important β-agonist is frequently found in pork to enhance food quality, which is harmful to human health. Thus, it is extremely important to develop an efficient analytical method to detect ractopamine for food safety control. Herein, we develop a kind of electrochemical biosensor using a biochar-supported Cu/Cu composite as an electrochemical sensing interface for detecting ractopamine. The electrochemical sensor combines the collective advantages of Nafion (enriches ractopamine and effectively shields the interference of negatively charged compounds), biochar (unique three-dimensional porous network structure to increase the contact area), and Cu/Cu (excellent electrical conductivity to speed up the charge transfer rate), which are beneficial to the accumulation and electrochemical sensing of targets on a Nafion-biochar-supported Cu/Cu electrode (NBC-GCE). The as-prepared sensor shows a good electrochemical sensing ability, with an ultralow detection limit and high sensitivity (0.041 μM and 416 μA·mM·cm, respectively), in the 0.1-1.75 μM range. In addition, the stability, repeatability, and anti-interference performance of the modified electrode are also satisfying. Last, its practicability is shown for assaying ractopamine in pork sausages. This research provides an environmentally friendly method to simultaneously treat food waste and achieve ultrasensitive detection of ractopamine in food.
莱克多巴胺作为一种重要的β-激动剂，常被添加到猪肉中以提高食品质量，但对人体健康有害。因此，开发一种高效的分析方法来检测莱克多巴胺以进行食品安全控制非常重要。在此，我们开发了一种基于生物炭负载的 Cu/Cu 复合材料的电化学生物传感器，用于检测莱克多巴胺。电化学传感器结合了 Nafion（富集莱克多巴胺并有效屏蔽带负电荷化合物的干扰）、生物炭（独特的三维多孔网络结构增加接触面积）和 Cu/Cu（优异的导电性以加快电荷转移速率）的集体优势，有利于目标在 Nafion-生物炭负载的 Cu/Cu 电极（NBC-GCE）上的积累和电化学传感。所制备的传感器在 0.1-1.75 μM 范围内表现出良好的电化学传感能力，具有超低的检测限和高灵敏度（分别为 0.041 μM 和 416 μA·mM·cm）。此外，修饰电极的稳定性、重复性和抗干扰性能也令人满意。最后，它在检测猪肉香肠中的莱克多巴胺方面显示出了实用性。这项研究提供了一种环保的方法，可同时处理食物垃圾并实现对食物中莱克多巴胺的超灵敏检测。
