Department of Chemistry, Hubei Engineering Center of Natural Polymers-based Medical Materials, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, China.
Department of Biomedical Engineering, Hubei Province Key Laboratory of Allergy and Immune Related Diseases, School of Basic Medical Science, Wuhan University, Wuhan 430071, China.
ACS Appl Bio Mater. 2021 Feb 15;4(2):1833-1842. doi: 10.1021/acsabm.0c01536. Epub 2021 Jan 11.
Chitin and chitosan have been extensively used as wound dressings because of their special functions to promote wound healing. However, there was little focus on the effects of the degree of acetylation (DA) on wound healing. In this work, the regenerated chitin nonwoven fabrics with DA values of 90, 71, 60, and 42% were prepared, and the morphology and physical performances of the fabrics were characterized. Moreover, the effects of DA of the chitin nonwoven fabrics on wound recovery were studied with a full-thickness skin defect model in rats. experiments indicated that the chitin nonwoven fabrics exhibited good biocompatibility and blood compatibility and a low blood-clotting index (BCI). experiments revealed that the chitin nonwoven fabrics could accelerate wound healing more effectively than gauze by promoting re-epithelialization and collagen deposition as well as by stimulating neovascularization. The results of the wound healing process showed that DA of the chitin nonwoven fabrics had a profound effect on promoting wound healing. Notably, the regenerated chitin nonwoven fabrics with 71% DA significantly improved the wound healing compared to the commercial wound dressing Algoplaque film. Therefore, the regenerated chitin nonwoven fabrics are promising candidates for wound healing.
壳聚糖和几丁聚糖由于其促进伤口愈合的特殊功能而被广泛用作伤口敷料。然而，对于乙酰化程度（DA）对伤口愈合的影响关注甚少。在这项工作中，制备了 DA 值分别为 90、71、60 和 42%的再生壳聚糖非织造布，并对其形貌和物理性能进行了表征。此外，还通过大鼠全层皮肤缺损模型研究了壳聚糖非织造布的 DA 对伤口恢复的影响。实验表明，壳聚糖非织造布具有良好的生物相容性和血液相容性，凝血指数（BCI）较低。实验表明，壳聚糖非织造布通过促进再上皮化和胶原沉积以及刺激新血管生成，比纱布更有效地加速伤口愈合。伤口愈合过程的结果表明，壳聚糖非织造布的 DA 对促进伤口愈合有深远的影响。值得注意的是，与商业伤口敷料 Algoplaque 膜相比，DA 为 71%的再生壳聚糖非织造布显著改善了伤口愈合。因此，再生壳聚糖非织造布是伤口愈合的有前途的候选材料。
