State Key Lab of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China.
Nanjing Customs District Light Industry Products and Children's Products Inspection Center, Yangzhou 225009, P. R. China.
ACS Appl Bio Mater. 2022 Jan 17;5(1):285-294. doi: 10.1021/acsabm.1c01079. Epub 2021 Dec 16.
Rapid detection of aflatoxin B1 (AFB1) is a very important task in food safety monitoring. However, it is still challenging to achieve highly sensitive detection without antibody or aptamer biomolecules. In this work, a rapid detection of aflatoxin B1 was achieved using a ratiometric fluorescence probe without antibody or aptamer for the first time. In the ratiometric fluorescence system, the fluorescence emission of AFB1 at 433 nm was significantly enhanced due to the β-cyclodextrin-AFB1 host-guest interaction and the complexation of AFB1 and Pt. Meanwhile, the inclusion of aflatoxin B1 also quenched the fluorescence emission of β-CD@Cu nanoparticles (NPs) at 650 nm based on inner filter effect mechanism. On the basis of the above effects, the ratiometric detection of aflatoxin B1 was achieved in the range of 0.03-10 ng/mL with a low detection limit of 0.012 ng/mL (3σ/s). In addition, the β-CD@Cu NPs based nanoprobe could achieve stable response within 1 min to AFB1. The above ratiometric detection also demonstrated excellent application potential in the rapid on-site detection of AFB1 in food due to the advantages of convenience, rapidness, and high accuracy.
快速检测黄曲霉毒素 B1（AFB1）是食品安全监测中非常重要的任务。然而，在没有抗体或适体生物分子的情况下，实现高灵敏度检测仍然具有挑战性。在这项工作中，首次使用无抗体或适体的比率荧光探针实现了对黄曲霉毒素 B1 的快速检测。在比率荧光体系中，由于β-环糊精-AFB1 主客体相互作用和 AFB1 与 Pt 的络合，AFB1 在 433nm 处的荧光发射显著增强。同时，基于内滤效应机制，黄曲霉毒素 B1 的包合也猝灭了β-CD@Cu 纳米粒子（NPs）在 650nm 处的荧光发射。基于上述效果，实现了 0.03-10ng/mL 范围内的黄曲霉毒素 B1 的比率检测，检测限低至 0.012ng/mL（3σ/s）。此外，β-CD@Cu NPs 基纳米探针可在 1 分钟内对 AFB1 做出稳定响应。由于具有方便、快速和高精度的优点，上述比率检测也展示了在食品中快速现场检测 AFB1 的巨大应用潜力。
