Jia Yongmei, Zhou Guohua, Wang Xudong, Zhang Yingzi, Li Zhiguo, Liu Peilian, Yu Biao, Zhang Jun
School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang, 524048, China.
School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang, 524048, China.
Talanta. 2020 Nov 1;219:121342. doi: 10.1016/j.talanta.2020.121342. Epub 2020 Jul 9.
The demand of simple, sensitive, selective and reliable assay for aflatoxin B1 (AFB1) detection is ubiquitous in food safety, due to its high toxic. Herein, a novel fluorescent aptasensor using metal-organic frameworks (UiO-66-NH) and TAMRA label aptamer as sensing platform for AFB1 detection was developed. The TAMRA aptamer adsorbed on the surface of UiO-66-NH via van der Waals force and its fluorescence was quenched for the charge transfer from fluorescence dye TAMRA to metal ions of UiO-66-NH. After introducing AFB1 to the system, the TAMRA aptamer binded to AFB1 and formed TAMRA aptamer/AFB1complex, making its conformation change and resulting in fluorescence recovery. Thus, the quantity of AFB1 could be analyzed according to the fluorescence signal change. Under optimize experimental conditions, the assay exhibited high sensitivity toward AFB1 in range of 0-180 ng mL with low limit of detection of 0.35 ng mL and good specificity against other toxins. Moreover, the aptamer/metal-organic frameworks sensing platform could be utilized to determine AFB1 content in food samples such as corn, rice and milk. It provided a reasonable method for other mycotoxin detection by changing the sequence of aptamer.
由于黄曲霉毒素B1（AFB1）毒性很强，因此在食品安全领域，对其进行简单、灵敏、选择性好且可靠的检测的需求十分普遍。在此，开发了一种新型荧光适配体传感器，该传感器以金属有机框架（UiO-66-NH）和TAMRA标记的适配体作为检测AFB1的传感平台。TAMRA适配体通过范德华力吸附在UiO-66-NH表面，由于荧光染料TAMRA向UiO-66-NH的金属离子发生电荷转移，其荧光被猝灭。向系统中引入AFB1后，TAMRA适配体与AFB1结合形成TAMRA适配体/AFB1复合物，使其构象发生变化，导致荧光恢复。因此，可以根据荧光信号变化分析AFB1的含量。在优化的实验条件下，该检测方法对0-180 ng/mL范围内的AFB1表现出高灵敏度，检测限低至0.35 ng/mL，并且对其他毒素具有良好的特异性。此外，适配体/金属有机框架传感平台可用于测定玉米、大米和牛奶等食品样品中的AFB1含量。通过改变适配体序列，它为其他霉菌毒素的检测提供了一种合理的方法。
