CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
Key Laboratory of Sensor and Sensing Technology of Gansu Province, Gansu Academy of Sciences, Lanzhou 730000, China.
ACS Appl Bio Mater. 2022 Jan 17;5(1):295-304. doi: 10.1021/acsabm.1c01086. Epub 2021 Dec 31.
Until now, the green and facile synthesis of multicolor fluorescent silicon nanoparticles (SiNPs) with favorable biocompatibility for cellular imaging and biosensors is still a challenge. Herein, a facile one-step room temperature method for preparing fluorescent SiNPs displayed different emission wavelengths was reported. Green and red fluorescent SiNPs (G-SiNPs and R-SiNPs) were synthesized by adjusting the concentration of the reducing agent 2,4-diaminophenol hydrochloride when the amount of N-[3-(trimethoxysilyl)-propyl]-ethylenediamine was consistent. Characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy, the results revealed that the G-SiNPs and R-SiNPs were assembled by polymerization of different building blocks, and the emission characteristics of these SiNPs were attributed to the difference in their structural composition and particle size. Interestingly, these fluorescent SiNPs exhibited excellent water solubility, salt tolerance, pH stability, photobleaching resistance, and low cytotoxicity, which facilitated multicolor cell imaging, and further led to these SiNPs were highly attractive in a variety of applications, such as multi-channel sensing and biological imaging. Furthermore, the R-SiNPs have shown the potential to detect acid phosphatase, which is a biomarker of prostate cancer.
到目前为止，用绿色简便的方法合成具有良好生物相容性的多色荧光硅纳米粒子（SiNPs），用于细胞成像和生物传感器仍然是一个挑战。本文报道了一种简便的一步室温法，用于制备显示不同发射波长的荧光 SiNPs。通过调整还原剂 2,4-二氨基苯酚盐酸盐的浓度，当 N-[3-(三甲氧基硅基)-丙基]-乙二胺的用量相同时，可以合成绿色和红色荧光 SiNPs（G-SiNPs 和 R-SiNPs）。傅里叶变换红外光谱、透射电子显微镜和 X 射线光电子能谱的结果表明，G-SiNPs 和 R-SiNPs 是由不同的结构单元聚合而成的，这些 SiNPs 的发光特性归因于它们的结构组成和粒径的差异。有趣的是，这些荧光 SiNPs 具有优异的水溶性、耐盐性、pH 稳定性、抗光漂白性和低细胞毒性，这有利于多色细胞成像，进一步使这些 SiNPs 在多种应用中具有吸引力，如多通道传感和生物成像。此外，R-SiNPs 已显示出检测酸性磷酸酶（前列腺癌的生物标志物）的潜力。
