School of Chemistry and Materials Science, Shanxi Normal University, TaiYuan, 030032, China.
Mikrochim Acta. 2024 Sep 12;191(10):592. doi: 10.1007/s00604-024-06660-2.
Highly stable copper nanocluster (CuNCs) with aggregation-induced emission (AIE) properties was synthesized. α-, β-, and γ- MnO were utilized as quenchers, with CuNCs fluorescence quenching of 48.9%, 91.5%, and 96.6%, respectively. L-ascorbate-2-phosphate (AAP) was hydrolyzed by acid phosphatase (ACP), and ascorbic acid (AA) was formed. Then, MnO could be restored by AA, and the fluorescence of the CuNCs could be restored. An on-off-on detection platform with a high signal/noise ratio was constructed for the sensing of ACP. The fluorescence recovery rate of the CuNCs was related to the crystal forms of MnO. Then, the equilibrium constants (K) for the reaction between AA and MnO were calculated to evaluate the reaction process. Compared with the K values of CuNCs/α-MnO and CuNCs/γ-MnO, the K values for AA and β-MnO were maximum. The CuNCs/β-MnO system exhibited optimal fluorescence recovery for the sensitive detection of ACP. In the concentration range 0.005-0.06 U/mL, the detection limit was 0.0028 U/mL. The determination of serum ACP levels also revealed satisfactory results. This study provides novel insights into enhancing the sensitivity of the determination of quenchers in different crystal form.
合成了具有聚集诱导发射（AIE）性质的高稳定性铜纳米团簇（CuNCs）。α-、β-和γ- 氧化锰（MnO）分别作为猝灭剂，CuNCs 的荧光猝灭率分别为 48.9%、91.5%和 96.6%。抗坏血酸-2-磷酸酯（AAP）被酸性磷酸酶（ACP）水解，形成抗坏血酸（AA）。然后，AA 可以还原 MnO，恢复 CuNCs 的荧光。构建了一个具有高信噪比的开-关-开检测平台，用于 ACP 的传感。CuNCs 的荧光恢复率与 MnO 的晶体形式有关。然后，计算了 AA 和 MnO 之间反应的平衡常数（K），以评估反应过程。与 CuNCs/α-MnO 和 CuNCs/γ-MnO 的 K 值相比，AA 和 β-MnO 的 K 值最大。CuNCs/β-MnO 体系对 ACP 的灵敏检测表现出最佳的荧光恢复。在 0.005-0.06 U/mL 的浓度范围内，检测限为 0.0028 U/mL。对血清 ACP 水平的测定也显示出令人满意的结果。本研究为提高不同晶体形态猝灭剂测定的灵敏度提供了新的思路。
