一种用于检测鼠粪便中 RNPP 型群体感应肽的生物分析筛选方法。
A bioanalytical screening method for RNPP-type quorum sensing peptides in murine feces.
机构信息
Drug Quality & Registration (DruQuaR) group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000, Ghent, Belgium.
出版信息
Bioanalysis. 2022 Feb;14(3):151-167. doi: 10.4155/bio-2021-0225. Epub 2022 Jan 11.
Bacteria coordinate their behavior as a group via communication with their peers, known as 'quorum sensing'. employs quorum sensing via RNPP-peptides which were not yet reported to be present in mammalian biofluids. Solid phase extraction of murine feces was performed, followed by ultra high performance liquid chromatography (UHPLC-MS/MS) in multiple reaction monitoring (MRM) mode (in total <90 min/sample) for the nine known RNPP peptides. Limits of detection ranged between 0.045 and 52 nM. Adequate identification criteria allowed detection of RNPP quorum sensing peptides in 2/20 wild-type murine feces samples (i.e., cAM373 and cOB1). A fit-for-purpose UHPLC-MS/MS method detected these RNPP peptides in wild-type murine feces samples.
细菌通过与同伴进行交流（称为“群体感应”）来协调它们的行为。 利用 RNPP-肽进行群体感应，而这些肽尚未在哺乳动物生物流体中报道过。 对鼠粪便进行固相萃取，然后采用超高效液相色谱-串联质谱（UHPLC-MS/MS）在多重反应监测（MRM）模式下（每个样品总共 <90 分钟）对 9 种已知的 RNPP 肽进行分析。检测限在 0.045 和 52 nM 之间。适当的鉴定标准允许在 2/20 个野生型鼠粪便样本中（即 cAM373 和 cOB1）检测到 RNPP 群体感应肽。 一种适合目的的 UHPLC-MS/MS 方法可以检测到野生型鼠粪便样本中的这些 RNPP 肽。
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