一种适用于分析人唾液中选定链球菌群体感应肽的 LC-MS/MS 方法。

A fit-for-purpose LC-MS/MS method for the analysis of selected Streptococcal quorum sensing peptides in human saliva.

机构信息

Drug Quality and Registration (DruQuaR) group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, Belgium.

Drug Quality and Registration (DruQuaR) group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, Belgium; Geriatrics Department, Faculty of Medicine, Ghent University, Corneel Heymanslaans 10, B-9000 Ghent, Belgium.

出版信息

J Pharm Biomed Anal. 2022 May 10;213:114594. doi: 10.1016/j.jpba.2022.114594. Epub 2022 Jan 15.

DOI:10.1016/j.jpba.2022.114594

PMID:35220201

Abstract

The development of analytical methods for the detection of peptides at the nanomolar level can be challenging. Peptides can suffer from adsorption, rendering the detection of peptides at these low levels difficult. A subset of peptides are the quorum sensing peptides, which are bacterial communication molecules demonstrating possible host effects as well. However, their direct presence in human biofluids has only rarely been reported. Therefore, a UHPLC-MS/MS method capable of detecting 15 selected Streptococcal competence stimulating quorum sensing peptides at the nanomolar level in human saliva was developed. This method, using an anti-adsorption diluent, was applied on saliva samples obtained from 38 healthy donors. Six donors did have a positive hit for at least one of three competence stimulating quorum sensing peptides using a triple quadrupole assay. These observations indicate that Streptococcus species produce quorum sensing peptides in the human oral cavity.

摘要

开发用于检测纳摩尔水平肽的分析方法可能具有挑战性。肽可能会受到吸附的影响，从而难以在这些低水平下检测到肽。肽的一个子集是群体感应肽，它们是细菌的通讯分子，也表现出可能对宿主的影响。然而，它们在人体生物液中的直接存在很少有报道。因此，开发了一种能够在人唾液中检测到 15 种选定的链球菌感应肽的纳摩尔水平的 UHPLC-MS/MS 方法。该方法使用抗吸附稀释剂，应用于从 38 名健康供体获得的唾液样本。使用三重四极杆分析，有 6 名供体至少有一种三刺激感应肽呈阳性。这些观察结果表明，链球菌属在人类口腔中产生群体感应肽。