Physical Therapy Department, Universidade Paulista (UNIP), Doutor Bacelar Street, 1.212, São Paulo, SP, Brazil.
Discipline of Experimental Surgery and Operative Technique, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Lasers Med Sci. 2022 Apr;37(3):1495-1501. doi: 10.1007/s10103-021-03303-3. Epub 2022 Jan 11.
The aim of this study was to investigate the effect of red laser (660 nm) photobiomodulation (PBM) with different energies on tumor necrosis factor-alpha(TNF-α) expression for random skin flap viability in rats. Twenty-four Wistar rats were divided into three groups: sham group (SG), PBM laser group with an energy dose of 0.29 J (0.29G), and PBM laser group with an energy dose of 7.30 J (7.30G). A cranially based dorsal skin flap measuring 10 × 4 cm was raised and a plastic barrier was placed between the flap and its bed. PBM was applied in 3 timepoints: in the immediate postoperative period, in the 1 and in the 2 postoperative days; the animals were euthanized on the 7 postoperative day. The assessments included: TNF-α expression of 3 different flap areas (proximal, medial and distal), by immunohistochemistry; percentage of skin flap necrosis area, by the paper template method. The statistical analysis was performed through the Kruskal-Wallis and Mann-Whitney tests, the level of significance adopted was 5% (p < 0.05). TNF-α expression was significantly lower for 7.30G in the proximal area, reduced for SG in the medial point, and larger for 7.30G in the distal area. The percentage of flap necrosis area was significantly reduced for 7.30G. Higher energy doses are more efficacious than lower energy doses for modulating TNF-α expression. PBM with an energy dose of 7.30 J was effective in reducing the expression of TNF-α and increase skin flap viability.
本研究旨在探讨不同能量的红光（660nm）光生物调节（PBM）对大鼠随意皮瓣存活的肿瘤坏死因子-α（TNF-α）表达的影响。将 24 只 Wistar 大鼠分为三组：假手术组（SG）、能量剂量为 0.29J（0.29G）的 PBM 激光组和能量剂量为 7.30J（7.30G）的 PBM 激光组。制备 10×4cm 的颅顶背侧皮瓣，在皮瓣与其床之间放置塑料屏障。PBM 在术后即刻、术后第 1 天和第 2 天共应用 3 次；术后第 7 天处死动物。评估包括：免疫组织化学法检测 3 个不同皮瓣区域（近端、中间和远端）的 TNF-α 表达；通过纸模板法评估皮瓣坏死面积百分比。采用 Kruskal-Wallis 和 Mann-Whitney 检验进行统计学分析，采用 5%（p<0.05）作为显著性水平。在近端区域，7.30G 组的 TNF-α 表达明显降低，SG 组的中间点表达减少，7.30G 组的远端区域表达增加。皮瓣坏死面积百分比显著降低。较高的能量剂量比较低的能量剂量更有效地调节 TNF-α 的表达。能量剂量为 7.30J 的 PBM 有效降低 TNF-α 的表达，增加皮瓣存活。
