Post Graduation Plastic Surgery, Federal University of São Paulo, R. Napoleão de Barros, 715, 4º andar, CEP 04024-900, São Paulo, SP, Brazil.
Lasers Med Sci. 2012 Sep;27(5):1045-50. doi: 10.1007/s10103-011-1042-7. Epub 2011 Dec 30.
Skin flap procedures are commonly used in plastic surgery. Failures can follow, leading to the necrosis of the flap. Therefore, many studies use LLLT to improve flap viability. Currently, the LED has been introduced as an alternative to LLLT. The objective of this study was to evaluate the effect of LLLT and LED on the viability of random skin flaps in rats. Forty-eight rats were divided into four groups, and a random skin flap (10 × 4 cm) was performed in all animals. Group 1 was the sham group; group 2 was submitted to LLLT 660 nm, 0.14 J; group 3 with LED 630 nm, 2.49 J, and group 4 with LLLT 660 nm, with 2.49 J. Irradiation was applied after surgery and repeated on the four subsequent days. On the 7th postoperative day, the percentage of flap necrosis was calculated and skin samples were collected from the viable area and from the transition line of the flap to evaluate blood vessels and mast cells. The percentage of necrosis was significantly lower in groups 3 and 4 compared to groups 1 and 2. Concerning blood vessels and mast cell numbers, only the animals in group 3 showed significant increase compared to group 1 in the skin sample of the transition line. LED and LLLT with the same total energies were effective in increasing viability of random skin flaps. LED was more effective in increasing the number of mast cells and blood vessels in the transition line of random skin flaps.
皮瓣手术在整形手术中很常见。手术可能会失败，导致皮瓣坏死。因此，许多研究使用低水平激光疗法来提高皮瓣的存活率。目前，已经引入了 LED 作为 LLLT 的替代方法。本研究的目的是评估 LLLT 和 LED 对大鼠随意皮瓣存活率的影响。将 48 只大鼠分为四组，所有动物均行随意皮瓣（10×4 cm）。第 1 组为假手术组；第 2 组接受 660nm 的 LLLT，0.14J；第 3 组采用 630nm 的 LED，2.49J，第 4 组采用 660nm 的 LLLT，2.49J。手术后进行照射，并在接下来的四天重复照射。术后第 7 天，计算皮瓣坏死的百分比，并从存活区和皮瓣过渡线采集皮肤样本，以评估血管和肥大细胞。与第 1 组和第 2 组相比，第 3 组和第 4 组的皮瓣坏死百分比明显降低。关于血管和肥大细胞数量，只有第 3 组的动物在过渡线的皮肤样本中与第 1 组相比有显著增加。具有相同总能量的 LED 和 LLLT 可有效提高随意皮瓣的存活率。LED 更有效地增加了随意皮瓣过渡线处的肥大细胞和血管数量。
