Atatürk University, Faculty of Sciences, Department of Chemistry, 25240-, Erzurum, Turkey.
Department of Biotechnology, Faculty of Science, Bartin University, 74100-, Bartin, Turkey.
Chem Biodivers. 2022 Mar;19(3):e202100775. doi: 10.1002/cbdv.202100775. Epub 2022 Jan 27.
In the present work, antioxidant and antidiabetic potentials of mountain mint [Cyclotrichium leu-cotrichum (Stapf ex Rech. Fil.) Leblebici] was the first time appraised. In this sense, methanol (MECL) and water (WECL) extracts were obtained from aerial parts of mountain mint (Cyclotrichium leucotrichum) and studied for their antioxidant ability by several bioanalytical assays. Also, their inhibition profiles were realized toward several metabolic enzymes connected to some diseases, including butyrylcholinesterase (BChE), α-glycosidase, acetylcholinesterase (AChE), and α-amylase enzymes. Additionally, their phenolic contents were determined by putative chromatographic method of LC/MS/MS. Consequently, nineteen phenolic molecules were identified in MECL and fifteen phenolic molecules were found in WECL. Also, antioxidant effects of both extracts were studied using by the methods of 1,1-diphenyl-2-picryl-hydrazyl (DPPH⋅), 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS ) and N,N-dimethyl-p-phenylenediamine (DMPD ) scavenging activities, ferric (Fe ) and cupric (Cu ) ions and Fe -2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) reducing capacities. MECL and WECL were found as powerful DPPH⋅ (IC : 23.74 and 28.85 μg/mL), ABTS (IC : 12.53 and 14.05 μg/mL) and DMPD scavenging effects (IC : 43.52 and 54.80 μg/mL). Also, both extracts demonstrated the effective inhibition on AChE (IC : 69.31 and 115.51 μg/mL), BChE (IC : 57.75 and 86.62 μg/mL), α-glycosidase (IC : 36.47 and 62.94 μg/mL) and α-amylase (IC : 1.01 and 3.43 μg/mL). This study will be useful for future studies to determine the antioxidant properties and enzyme inhibition profile of food, medical and industrially important plants.
本研究首次评估了山薄荷（Cyclotrichium leucotrichum(Stapf ex Rech. Fil.) Leblebici）的抗氧化和抗糖尿病潜力。为此，从山薄荷（Cyclotrichium leucotrichum）的地上部分提取甲醇（MECL）和水（WECL）提取物，并通过几种生物分析方法评估其抗氧化能力。此外，还研究了它们对几种与疾病相关的代谢酶（包括丁酰胆碱酯酶（BChE）、α-糖苷酶、乙酰胆碱酯酶（AChE）和α-淀粉酶）的抑制作用。此外，还通过可能的 LC/MS/MS 色谱方法测定了它们的酚类含量。结果，在 MECL 中鉴定出 19 种酚类分子，在 WECL 中鉴定出 15 种酚类分子。此外，还通过 1,1-二苯基-2-苦基肼（DPPH⋅）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和 N,N-二甲基-p-苯二胺（DMPD）清除活性、铁（Fe）和铜（Cu）离子和 Fe-2,4,6-三（2-吡啶基）-s-三嗪（TPTZ）还原能力研究了两种提取物的抗氧化作用。发现 MECL 和 WECL 具有强大的 DPPH⋅（IC：23.74 和 28.85μg/mL）、ABTS（IC：12.53 和 14.05μg/mL）和 DMPD 清除作用（IC：43.52 和 54.80μg/mL）。此外，两种提取物均能有效抑制 AChE（IC：69.31 和 115.51μg/mL）、BChE（IC：57.75 和 86.62μg/mL）、α-糖苷酶（IC：36.47 和 62.94μg/mL）和α-淀粉酶（IC：1.01 和 3.43μg/mL）。本研究将有助于未来研究确定食品、医学和工业上重要植物的抗氧化特性和酶抑制谱。
