D. Royen ex L 茎叶水提物对 Wistar 大鼠铅诱导的睾丸毒性具有保护作用。
Aqueous extract of D. Royen ex L leafy stem protects against lead induced testicular toxicity in Wistar rats.
机构信息
Experimental Physiology and Pharmacology Laboratory, Faculty of Sciences and Technology, University of Abomey-Calavi, Cotonou Benin, Benin.
Histology, Biology of Reproduction, Cytogenetics and Medical Genetics Laboratory, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou Benin, Benin.
出版信息
J Complement Integr Med. 2022 Jan 10;19(3):683-690. doi: 10.1515/jcim-2021-0482. eCollection 2022 Sep 1.
OBJECTIVES
Lead exposure seriously impairs male reproductive function. The protective capacity of leafy stem and fruit aqueous extracts against lead testicular toxicity is evaluated to find herbals drugs able to improve semen quality.
METHODS
Phytochemical screening were performed according to classical methods. Twenty four male rats were divided into four groups of six rats each and received the following treatments via oral route: distilled water; 0.2% lead acetate in drinking water; 0.2% lead acetate in drinking water with 400 mg/kg aqueous leafy stem extract; 0.2% leaded water with 400 mg/kg aqueous fruit extract. Treatments were administered for 70 days. Body and reproductive organs weights, sperm parameters and testicular histological sections of each group were examined.
RESULTS
Flavonoids, tannins, coumarins, alkaloids, and lignans were found in both extracts. Lead intoxication reduced sperm motility and count but increased the percentage of morphologically abnormal sperms. The germinal epithelium of seminiferous tubules histoarchitecture was disorganized by lead. The leafy stem extract was effective in reducing lead induced testicular disruption whereas fruit has not shown any beneficial effect.
CONCLUSIONS
leafy stem aqueous extract is effective against semen alterations caused by lead.
目的
铅暴露严重损害男性生殖功能。评估茎叶和果实水提物对铅睾丸毒性的保护作用，以寻找能够改善精液质量的草药药物。
方法
根据经典方法进行植物化学筛选。将 24 只雄性大鼠分为四组，每组 6 只，通过口服途径接受以下治疗：蒸馏水；饮用水中的 0.2%醋酸铅；饮用水中的 0.2%醋酸铅和 400mg/kg 茎叶水提物；含 400mg/kg 水提物的水果。治疗持续 70 天。检查每组的体重、生殖器官重量、精子参数和睾丸组织学切片。
结果
两种提取物均含有类黄酮、单宁、香豆素、生物碱和木脂素。铅中毒降低了精子活力和数量，但增加了形态异常精子的百分比。铅使生精小管的生殖上皮组织形态结构紊乱。茎叶提取物可有效减轻铅引起的睾丸损伤，而果实提取物则没有显示出任何有益作用。
结论
茎叶水提物可有效对抗铅引起的精液改变。
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