State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, China.
Prep Biochem Biotechnol. 2022;52(9):990-1000. doi: 10.1080/10826068.2021.2023822. Epub 2022 Jan 11.
This study aimed to elucidate the molecular mechanisms through which succinic acid and fluconazole stimulate pigment biosynthesis under liquid fermentation conditions. The pigment yield was significantly improved by adding 0.35 g·L succinic acid or 1.5 g·L fluconazole. Transcriptome sequencing and RT-qPCR confirmation were performed to reveal transcriptome changes. The results indicated that the addition of succinic acid significantly decreased mRNA expression of genes involved in fatty acid biosynthesis while increasing expression of genes involved in pyruvate metabolism. Fluconazole significantly down-regulated transcripts involved in branched-chain amino acid metabolism, fatty acid metabolism, glycolysis/gluconeogenesis, and pyruvate metabolism, as well as the generation of acetyl-CoA for pigment biosynthesis. On the other hand, nitrogen metabolism and lysine degradation pathways were significantly enriched, which could stimulate the generation of acetyl-CoA. Therefore, the mechanism for enhancing pigment yield may be attributed to the competitive regulation of metabolic pathways toward acetyl-CoA biosynthesis. Additionally, up-regulation of some different key genes in the presence of fluconazole or succinic acid was involved in improving pigment production. This study deepens the theoretical understanding for enhancing pigment biosynthesis and provides a few potential approaches for improving pigment yield.
本研究旨在阐明琥珀酸和氟康唑在液体发酵条件下刺激色素生物合成的分子机制。在添加 0.35g·L 的琥珀酸或 1.5g·L 的氟康唑后，色素产量显著提高。通过转录组测序和 RT-qPCR 验证来揭示转录组变化。结果表明，添加琥珀酸可显著降低参与脂肪酸生物合成的基因的 mRNA 表达，同时增加参与丙酮酸代谢的基因的表达。氟康唑显著下调参与支链氨基酸代谢、脂肪酸代谢、糖酵解/糖异生和丙酮酸代谢以及乙酰辅酶 A 生成的色素生物合成的转录本。另一方面，氮代谢和赖氨酸降解途径显著富集，这可能刺激乙酰辅酶 A 的生成。因此，提高色素产量的机制可能归因于代谢途径对乙酰辅酶 A 生物合成的竞争调节。此外，氟康唑或琥珀酸存在时某些不同关键基因的上调参与了提高色素产量。本研究深化了对增强色素生物合成的理论认识，并为提高色素产量提供了一些潜在的方法。
