Liu Huiqian, Zhang Mengyao, Xu Linlin, Xue FuRong, Chen Wei, Wang Chengtao
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing, 100048, PR China.
Heliyon. 2024 May 22;10(11):e31619. doi: 10.1016/j.heliyon.2024.e31619. eCollection 2024 Jun 15.
Exploring the symbiotic potential between fungal and yeast species, this study investigates the co-cultivation dynamics of , a prolific producer of pharmacologically relevant secondary metabolites, and . The collaborative interaction between these microorganisms catalyzed a substantial elevation in the biosynthesis of secondary metabolites, prominently Monacolin K and natural pigments. Central to our discoveries was the identification and enhanced production of oxylipins (13S-hydroxyoctadecadienoic acid，13S-HODE), putative quorum-sensing molecules, within the co-culture environment. Augmentation with exogenous oxylipins not only boosted Monacolin K production by over half but also mirrored morphological adaptations in , affecting both spores and mycelial structures. This augmentation was paralleled by a significant upregulation in the transcriptional activity of genes integral to the Monacolin K biosynthetic pathway, as well as genes implicated in pigment and spore formation. Through elucidating the interconnected roles of quorum sensing, G-protein-coupled receptors, and the G-protein-mediate signaling pathway, this study provides a comprehensive view of the molecular underpinnings facilitating these metabolic enhancements. Collectively, our findings illuminate the profound influence of co-culture on , advocating for oxylipins as a pivotal quorum-sensing mechanism driving the observed symbiotic benefits.
本研究探索真菌与酵母物种之间的共生潜力，调查了作为药理学相关次生代谢产物丰富生产者的[具体物种1]与[具体物种2]的共培养动态。这些微生物之间的协同相互作用催化了次生代谢产物生物合成的显著提高，尤其是莫纳可林K和天然色素。我们发现的核心是在共培养环境中鉴定并增强了氧化脂质（13S-羟基十八碳二烯酸，13S-HODE）的产生，氧化脂质被认为是群体感应分子。用外源性氧化脂质进行补充不仅使莫纳可林K的产量提高了一半以上，还反映了[具体物种1]的形态适应性变化，影响了孢子和菌丝体结构。这种增加与莫纳可林K生物合成途径中不可或缺的基因以及与色素和孢子形成相关的基因的转录活性显著上调同时发生。通过阐明群体感应、G蛋白偶联受体和G蛋白介导的信号通路之间的相互关联作用，本研究全面揭示了促进这些代谢增强的分子基础。总体而言，我们的研究结果阐明了[具体物种1]与[具体物种2]共培养的深远影响，倡导将氧化脂质作为驱动所观察到的共生益处的关键群体感应机制。
