Ji Chendong, Ma Le, Chen Hongtao, Cai Yang, Zhao Xujie, Yin Meizhen
State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Key Laboratory of Biomedical Materials of Natural Macromolecules, Beijing University of Chemical Technology, 100029 Beijing, China.
ACS Appl Bio Mater. 2019 Jan 22;2(1):555-561. doi: 10.1021/acsabm.8b00699. Epub 2018 Dec 24.
Formaldehyde (FA), as a reactive carbonyl species, is extremely hazardous to human health if its concentration is above normal level. In live cells, lysosome is a main organelle to generate endogenous FA. Thus, the design of facile, stable, and sensitive probes for the detection of FA in lysosome is essential. Herein, a self-assembled fluorescent nanoprobe based on homoallylamino substituted perylene (P-FA) has been developed for FA detection in lysosome. P-FA can react with FA along with emission color change from blue to green. P-FA exhibited high sensitivity and selectivity to FA in DMSO solution. In aqueous solution, P-FA self-assembled into uniform sphere-like nanoparticle as a fluorescent nanoprobe. Furthermore, the reaction between the nanoprobe and FA was greatly facilitated at pH 4-5, which led to a lower detection limit (0.96 μM at pH 5) than that in DMSO. In live cells, P-FA nanoprobe achieved long-term tracking of lysosome (over 12 h). The fluorescent nanoprobe was then used for both exogenous and endogenous FA detection. Our work provides a facile and effective strategy for the detection of FA in lysosome.
甲醛（FA）作为一种活性羰基化合物，如果其浓度高于正常水平，对人体健康危害极大。在活细胞中，溶酶体是产生内源性FA的主要细胞器。因此，设计用于检测溶酶体中FA的简便、稳定且灵敏的探针至关重要。在此，基于高烯丙基氨基取代苝（P-FA）开发了一种自组装荧光纳米探针，用于溶酶体中FA的检测。P-FA可与FA反应，同时发射颜色从蓝色变为绿色。P-FA在二甲基亚砜溶液中对FA表现出高灵敏度和选择性。在水溶液中，P-FA自组装成均匀的球状纳米颗粒作为荧光纳米探针。此外，纳米探针与FA之间的反应在pH 4-5时大大加速，这导致检测限（pH 5时为0.96 μM）低于在二甲基亚砜中的检测限。在活细胞中，P-FA纳米探针实现了对溶酶体的长期追踪（超过12小时）。然后，该荧光纳米探针用于外源性和内源性FA的检测。我们的工作为溶酶体中FA的检测提供了一种简便有效的策略。
