一种用于活细胞中甲醛特异性检测与成像的溶酶体靶向荧光探针。
A lysosome-targeted fluorescent probe for the specific detection and imaging of formaldehyde in living cells.
作者信息
Cai Songtao, Liu Chang, Gong Jin, He Song, Zhao Liancheng, Zeng Xianshun
机构信息
Tianjin Key Laboratory for Photoelectric Materials and Devices, and Key Laboratory of Display Materials & Photoelectric Devices, Ministry of Education, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China; School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
Tianjin Key Laboratory for Photoelectric Materials and Devices, and Key Laboratory of Display Materials & Photoelectric Devices, Ministry of Education, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Jan 15;245:118949. doi: 10.1016/j.saa.2020.118949. Epub 2020 Sep 17.
We presented herien the rational design, synthesis, and photophysical property studies of the lysosome-targeted fluorescence FA probe NP-Lyso, an isopropyl group modified ortho-diaminonaphthalimide derivative. After the reaction of FA and ortho-phenylenediamine modified with the isopropyl group in NP-Lyso, the probe exhibited favorable features such as a large fluorescence enhancement, specific selectivity and high sensitivity for the detection of FA. More importantly, NP-Lyso could be used to detect and image endogenous FA in lysosomes. In light of these prominent properties, we envision that NP-Lyso will be an efficient optical imaging approach for investigating the biofunctions of FA in living systems.
我们在此展示了溶酶体靶向荧光叶酸（FA）探针NP-Lyso的合理设计、合成及光物理性质研究，NP-Lyso是一种异丙基修饰的邻二氨基萘二甲酰亚胺衍生物。在NP-Lyso中，FA与经异丙基修饰的邻苯二胺反应后，该探针表现出诸如荧光大幅增强、特异性选择性及对FA检测的高灵敏度等良好特性。更重要的是，NP-Lyso可用于检测溶酶体内的内源性FA并进行成像。鉴于这些突出特性，我们设想NP-Lyso将成为一种用于研究活体内FA生物功能的高效光学成像方法。
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