Mallick Abhik, Nandi Aditi, Basu Sudipta
Department of Chemistry, Indian Institute of Science Education and Research (IISER)-Pune, Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra 411008, India.
Discipline of Chemistry, Indian Institute of Technology (IIT)-Gandhinagar, Palaj, Gandhinagar, Gujarat 382355, India.
ACS Appl Bio Mater. 2019 Jan 22;2(1):14-19. doi: 10.1021/acsabm.8b00519. Epub 2018 Dec 31.
Mitochondrion, the powerhouse of the cells, controls bioenergetics, biosynthesis, metabolism, and signaling. Consequently, it has become an unorthodox target for cancer therapeutics. However, specific targeting of mitochondria into subcellular milieu in cancer cells remains a major challenge. To address this, we have engineered polyethylenimine cloaked positively charged self-assembled graphene oxide nanoparticle (PEI-GTC-NP) comprising topotecan and cisplatin concurrently. These PEI-GTC-NPs effectively homed into mitochondria in HeLa cervical cancer cells at 6 h and impaired mitochondria leading to reactive oxygen species generation followed by remarkably improved cancer cell death. This platform can be used for specific subcellular organelle targeting for future cancer therapy.
线粒体作为细胞的动力源，控制着生物能量学、生物合成、新陈代谢和信号传导。因此，它已成为癌症治疗中一个非传统的靶点。然而，将线粒体特异性靶向癌细胞的亚细胞环境仍然是一个重大挑战。为了解决这个问题，我们设计了同时包含拓扑替康和顺铂的聚乙烯亚胺包覆的带正电自组装氧化石墨烯纳米颗粒（PEI-GTC-NP）。这些PEI-GTC-NPs在6小时时有效地归巢到HeLa宫颈癌细胞的线粒体中，并损害线粒体，导致活性氧的产生，随后癌细胞死亡显著增加。该平台可用于未来癌症治疗的特定亚细胞器靶向。
