Durgadevi Sabapathi, Kumar Ponnuchamy
Food Chemistry and Molecular Cancer Biology, Department of Animal Health and Management, Alagappa University, 630 003, Karaikudi, Tamil Nadu, India.
Sci Rep. 2025 Aug 13;15(1):29737. doi: 10.1038/s41598-025-00983-7.
Herein, we report graphene nanosheets fabricated using liquid-phase exfoliation of graphite employing cetyltrimethylammonium chloride (CTAC) in an aqueous medium. The salient features of CTAC-assisted graphene nanosheets are as follows: (i) the concentration of graphene dispersed in water is found to be 26.01 mg/mL at 50 mg with a Critical Micelle Concentration (CMC) Value of 3 mg/mL (9.375 mM); (ii) rapid exfoliation is assisted by CTAC via sonication, which develops hydrophilic micelles on graphitic surface; (iii) the strong repulsive force of CTAC prevents re-aggregation of graphene sheets by means of electrostatic interaction as revealed by zeta potential measurement (+ 33.8 mV); (iv) the graphene nanosheets display excellent anticancer properties against MCF-7 (IC - 205.5 ng/mL) and MDA-MB-231 (IC - 216.9 ng/mL) breast cancer cells; (v) the CTAC assisted graphene nanosheets induce PI3K/Akt mediated apoptosis in breast cancer cells. Based on the results, CTAC-assisted graphene nanosheets are expected to be a potential candidate for therapeutic applications in the near future.
在此，我们报告了在水介质中使用十六烷基三甲基氯化铵（CTAC）通过石墨的液相剥离制备的石墨烯纳米片。CTAC辅助的石墨烯纳米片的显著特征如下：（i）在50mg时，分散在水中的石墨烯浓度为26.01mg/mL，临界胶束浓度（CMC）值为3mg/mL（9.375mM）；（ii）CTAC通过超声处理辅助快速剥离，在石墨表面形成亲水性胶束；（iii）如ζ电位测量（+33.8mV）所示，CTAC的强排斥力通过静电相互作用防止石墨烯片重新聚集；（iv）石墨烯纳米片对MCF-7（IC - 205.5ng/mL）和MDA-MB-231（IC - 216.9ng/mL）乳腺癌细胞显示出优异的抗癌特性；（v）CTAC辅助的石墨烯纳米片在乳腺癌细胞中诱导PI3K/Akt介导的凋亡。基于这些结果，预计CTAC辅助的石墨烯纳米片在不久的将来有望成为治疗应用的潜在候选者。
