Izuta Shin, Yamaguchi Satoshi, Kosaka Takahiro, Okamoto Akimitsu
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
ACS Appl Bio Mater. 2019 Jan 22;2(1):33-38. doi: 10.1021/acsabm.8b00656. Epub 2019 Jan 7.
Spatiotemporal control of cell-material interactions contributes to our understanding of cell biology and the development of cell engineering. Here, we first report the reversible and spatio-selective immobilization of nonadherent cells through the use of photoswitchable polymeric materials. The substrate coated with spiropyran-conjugated poly(ethylene glycol) (PEG) lipids, which bind to cell membranes via the lipid moiety only in their merocyanine form, enabled rapid cell immobilization and release in an on-off manner by irradiation with ultraviolet and visible light, respectively. Our work has the potential to improve the performance of cell manipulations on chips and to enable rapid cell arrangement/sorting on various surfaces.
细胞与材料相互作用的时空控制有助于我们对细胞生物学的理解以及细胞工程的发展。在此，我们首次报道了通过使用光开关聚合物材料对非粘附细胞进行可逆和空间选择性固定。涂有螺吡喃共轭聚乙二醇（PEG）脂质的底物，其仅以部花青形式通过脂质部分与细胞膜结合，通过分别用紫外光和可见光照射，能够以开-关方式快速固定和释放细胞。我们的工作有潜力提高芯片上细胞操作的性能，并能够在各种表面上快速进行细胞排列/分选。
