Kollarigowda Ravichandran H, Mathews Anu Stella, Abraham Sinoj
ACS Appl Bio Mater. 2019 Jan 22;2(1):277-283. doi: 10.1021/acsabm.8b00595. Epub 2018 Dec 18.
In this work, we fabricated a thermosensitive hydrogel based on NIPAM that incorporated an oxygen-permeable hyaluronic acid (HA) polysaccharide unit. The interpenetrating network (IPN) of NIPAM-HA-MA hydrogel was fabricated by using an ultraviolet (UV) cross-linking process. Importantly, we explored the real behavior kinetics of the hydrogel via the mechanical properties of Young's modulus and the shear strain. The mechanical properties were reduced when the temperature was raised from 25 to 35 °C, and lower temperatures increased the rigidity of the IPN gel. This dynamic change occurred because of NIPAM's thermosensitive character. Further, IPN hydrogel exhibits good transparency with the incorporation of MA-HA and this stimuli-responsive hydrogel was biocompatible with a human dermal fibroblasts cell line (HDF). The cells adherence was densest when the temperature reached 37 °C and was reduced when the temperature was lowered to 25 °C. We envision using such hydrogels as mimics of cell-instructive biomaterials in tissue engineering applications.
在这项工作中，我们制备了一种基于N-异丙基丙烯酰胺（NIPAM）的热敏水凝胶，其中包含一个透氧的透明质酸（HA）多糖单元。NIPAM-HA-MA水凝胶的互穿网络（IPN）是通过紫外线（UV）交联工艺制备的。重要的是，我们通过杨氏模量和剪切应变的力学性能探索了水凝胶的实际行为动力学。当温度从25℃升高到35℃时，力学性能降低，而较低的温度增加了IPN凝胶的刚性。这种动态变化是由于NIPAM的热敏特性所致。此外，IPN水凝胶通过掺入MA-HA表现出良好的透明度，并且这种刺激响应性水凝胶与人类皮肤成纤维细胞系（HDF）具有生物相容性。当温度达到37℃时细胞粘附最密集，而当温度降至25℃时细胞粘附减少。我们设想在组织工程应用中使用这种水凝胶作为具有细胞指导作用的生物材料的模拟物。
