Department of Microbiology, Immunology and Transplantation, University of Leuven, Leuven, Belgium.
Department of Microbiology, Immunology and Transplantation, University of Leuven, Leuven, Belgium; Department of Laboratory Medicine, OLV Hospital, Aalst, Belgium.
Clin Chim Acta. 2022 Mar 1;528:34-43. doi: 10.1016/j.cca.2021.12.029. Epub 2022 Jan 10.
Antibodies to dsDNA are an important laboratory parameter for diagnosis, monitoring and classification of systemic lupus erythematosus (SLE). In clinical laboratories, several techniques are used to detect and quantify anti-dsDNA antibodies. Each technique has its advantages and disadvantages regarding sensitivity, specificity, avidity and assay procedure. Assays differ with respect to the antigen source (native versus synthetic versus molecular biological) used and the way the antigen is presented (e.g. in solution, covalently linked to a solid phase,…). Consequently, correlation between assays can be poor and standardization of anti-dsDNA antibody tests is challenging. We here provide an overview of the currently available anti-dsDNA tests frequently used in clinical laboratories [Crithidia luciliae immunofluorescence test (CLIFT), Enzyme linked immune sorbent assay (ELISA), fluoroenzyme immunoassay (FEIA), chemiluminisence immunoassay (CIA), multiplexed bead-based assays and Farr-RIA] and their performance characteristics. From this literature study, we concluded that performance characteristics differ between assays. Often, a combination of techniques is necessary for the best result interpretation.
抗双链 DNA 抗体是诊断、监测和系统性红斑狼疮（SLE）分类的重要实验室参数。在临床实验室中，有几种技术用于检测和定量抗双链 DNA 抗体。每种技术在灵敏度、特异性、亲和力和检测程序方面都有其优缺点。检测方法在使用的抗原来源（天然、合成或分子生物学）和抗原呈现方式（例如溶液中、共价连接到固相上等）上有所不同。因此，检测之间的相关性可能较差，抗双链 DNA 抗体检测的标准化具有挑战性。在这里，我们提供了临床实验室中常用的当前可用的抗双链 DNA 检测方法（克氏锥虫免疫荧光试验（CLIFT）、酶联免疫吸附试验（ELISA）、荧光酶免疫测定（FEIA）、化学发光免疫测定（CIA）、基于多重珠的检测方法和 Farr-RIA）及其性能特征的概述。从这项文献研究中，我们得出结论，不同检测方法的性能特征不同。通常，需要结合多种技术才能得出最佳的结果解释。
