College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Toxicol Appl Pharmacol. 2022 Feb 1;436:115882. doi: 10.1016/j.taap.2022.115882. Epub 2022 Jan 10.
Oocyte maturation is essential for fertilization and early embryo development, and proper organelle functions guarantee this process to maintain high-quality oocytes. The type B trichothecene nivalenol (NIV) is a mycotoxin produced by Fusarium oxysporum and is commonly found in contaminated food. NIV intake affect growth, the immune system, and the female reproductive system. Here, we investigated NIV toxicity on mouse oocyte quality. Transcriptome analysis results showed that NIV exposure altered the expression of multiple genes involved in spindle formation and organelle function in mouse oocytes, indicating its toxicity on mouse oocyte maturation. Further analysis indicated that NIV exposure disrupted spindle structure and chromosome alignment, possibly through tubulin acetylation. NIV exposure induced aberrant mitochondria distribution and reduced mitochondria number, mitochondria membrane potential (MMP), and ATP levels. In addition, NIV caused the abnormal distribution of the Golgi apparatus and altered the expression of the vesicle trafficking protein Rab11. ER distribution was also disturbed under NIV exposure, indicating the effects of NIV on protein modification and transport in oocytes. Thus, our results demonstrated that NIV exposure affected spindle structure and organelles function in mouse oocytes.
卵母细胞成熟对于受精和早期胚胎发育至关重要，而适当的细胞器功能则保证了这一过程能够维持高质量的卵母细胞。B 型单端孢霉烯类毒素——雪腐镰刀菌烯醇（NIV）是由尖孢镰刀菌产生的一种真菌毒素，通常存在于污染的食物中。NIV 的摄入会影响生长、免疫系统和女性生殖系统。在这里，我们研究了 NIV 对小鼠卵母细胞质量的毒性作用。转录组分析结果表明，NIV 暴露改变了小鼠卵母细胞中多个参与纺锤体形成和细胞器功能的基因的表达，表明其对小鼠卵母细胞成熟具有毒性作用。进一步分析表明，NIV 暴露破坏了纺锤体结构和染色体排列，这可能是通过微管蛋白乙酰化实现的。NIV 暴露还导致线粒体分布异常，线粒体数量、线粒体膜电位（MMP）和 ATP 水平降低。此外，NIV 导致高尔基体的异常分布，并改变了囊泡运输蛋白 Rab11 的表达。内质网（ER）在 NIV 暴露下的分布也受到干扰，这表明 NIV 对卵母细胞中的蛋白质修饰和运输有影响。因此，我们的结果表明，NIV 暴露影响了小鼠卵母细胞中的纺锤体结构和细胞器功能。
