Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei 230022, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, No.81 Meishan Road, Hefei 230032, China; Key Laboratory of Population Health Across Life Cycle, Anhui Medical University, Ministry of Education of the People's Republic of China, No.81 Meishan Road, Hefei 230032, China; Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Bengbu Medical University, No.287 Changhuai Road, Bengbu 233000, China.
Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei 230022, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, No.81 Meishan Road, Hefei 230032, China; Key Laboratory of Population Health Across Life Cycle, Anhui Medical University, Ministry of Education of the People's Republic of China, No.81 Meishan Road, Hefei 230032, China.
Reprod Toxicol. 2024 Oct;129:108667. doi: 10.1016/j.reprotox.2024.108667. Epub 2024 Jul 24.
Bisphenol M (BPM), an alternative to bisphenol A (BPA), is commonly utilized in various industrial applications. However, BPM does not represent a safe substitute for BPA due to its detrimental effects on living beings. This research aimed to assess the influence of BPM exposure on the in vitro maturation of mouse oocytes. The findings revealed that BPM exposure had a notable impact on the germinal vesicle breakdown (GVBD) rate and polar body extrusion (PBE) rate throughout the meiotic progression of mouse oocytes, ultimately resulting in meiotic arrest. Investigations demonstrated that oocytes exposure to BPM led to continued activation of spindle assembly checkpoint. Further studies revealed that securin and cyclin B1 could not be degraded in BPM-exposed oocytes, and meiosis could not realize the transition from the MI to the AI stage. Mechanistically, BPM exposure resulted in abnormal spindle assembly and disrupted chromosome alignment of oocytes. Additionally, abnormal positioning of microtubule organizing center-associated proteins implied that MTOC may be dysfunctional. Furthermore, an elevation in the acetylation level of α-tubulin in oocytes was observed after BPM treatment, leading to decreased microtubule stability. In addition to its impact on microtubules, BPM exposure led to a reduction in the expression of the actin, signifying the disruption of actin assembly. Further research indicated a heightened incidence of DNA damage in oocytes following BPM exposure. Besides, BPM exposure induced alterations in histone modifications. The outcomes of this experiment demonstrate that BPM exposure impairs oocyte quality and inhibits meiotic maturation of mouse oocytes.
双酚 M（BPM）是双酚 A（BPA）的替代品，广泛应用于各种工业应用中。然而，由于 BPM 对生物的有害影响，它并不是 BPA 的安全替代品。本研究旨在评估 BPM 暴露对小鼠卵母细胞体外成熟的影响。研究结果表明，BPM 暴露对小鼠卵母细胞减数分裂进程中的核膜破裂（GVBD）率和第一极体排出（PBE）率有显著影响，最终导致减数分裂阻滞。研究表明，卵母细胞暴露于 BPM 会导致纺锤体组装检查点持续激活。进一步的研究表明，securin 和 cyclin B1 不能在 BPM 暴露的卵母细胞中降解，减数分裂不能从 MI 阶段过渡到 AI 阶段。从机制上讲，BPM 暴露导致卵母细胞纺锤体组装异常和染色体排列紊乱。此外，微管组织中心相关蛋白的异常定位表明 MTOC 可能功能失调。此外，BPM 处理后卵母细胞中α-微管蛋白的乙酰化水平升高，导致微管稳定性降低。除了对微管的影响外，BPM 暴露还导致肌动蛋白表达减少，表明肌动蛋白组装受到破坏。进一步的研究表明，BPM 暴露后卵母细胞中 DNA 损伤的发生率增加。此外，BPM 暴露还诱导组蛋白修饰的改变。该实验的结果表明，BPM 暴露会损害卵母细胞质量并抑制小鼠卵母细胞的减数分裂成熟。
