Wang Jilian, Li Mingyuan, Zhou Qian, Zhang Tian
Department of Biologic and Geographic Sciences, Kashi University, Kashi, China.
Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Kashi, China.
Front Microbiol. 2023 Jan 9;13:1086334. doi: 10.3389/fmicb.2022.1086334. eCollection 2022.
The continuous cropping of crops can result in the deterioration of the soil environment and cause a decline in plant health and yield, which complicates agricultural production. However, the effects of continuous melon cropping on rhizospheric microbial communities remain poorly understood.
In this study, high-throughput absolute quantification 16S rRNA gene amplicon sequencing was employed to analyze the bacterial community structure of greenhouse rhizosphere soil from Jiashi muskmelon replanted for 0, 1, 2, and 3 years (CK, 1a, 2a, and 3a, respectively).
The results showed that long- term continuous cropping caused significant changes in soil physicochemical properties. The bacterial absolute abundances increased, but the bacterial community richness and diversity were significantly lost (p < 0.05). The composition of bacterial community was more similar after 2 and 3 years of continuous cropping. The longer the continuous cropping years were, the greater the shift in the bacterial diversity and abundance. Analysis of potential functional components revealed that different bacterial groups were enriched in different continuous cropping years. The significant reduction of the taxa associated with nitrate reduction may be responsible for the loss of soil nitrogen in continuous cropping soil.
In summary, continuous cropping had a significant impact on the bacterial community structure of Jiashi muskmelon rhizospheric soil, and these results will provide a reference for soil management and scientific fertilization of melon and other crops under a continuous cropping regime.
作物连作会导致土壤环境恶化，致使植株健康状况和产量下降，这使农业生产变得复杂。然而，连作对甜瓜根际微生物群落的影响仍知之甚少。
本研究采用高通量绝对定量16S rRNA基因扩增子测序技术，分析了伽师甜瓜连作0、1、2和3年（分别为CK、1a、2a和3a）的温室根际土壤细菌群落结构。
结果表明，长期连作导致土壤理化性质发生显著变化。细菌绝对丰度增加，但细菌群落丰富度和多样性显著丧失（p < 0.05）。连作2年和3年后细菌群落组成更为相似。连作年限越长，细菌多样性和丰度的变化越大。对潜在功能成分的分析表明，不同细菌类群在不同连作年份中富集。与硝酸盐还原相关的分类群显著减少可能是连作土壤中土壤氮素损失的原因。
综上所述，连作对伽师甜瓜根际土壤细菌群落结构有显著影响，这些结果将为连作制度下甜瓜及其他作物的土壤管理和科学施肥提供参考。
