Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Laboratory of Microbiology and Virology, IRCCS San Raffaele Scientific Institute, Milan, Italy.
J Glob Antimicrob Resist. 2022 Mar;28:146-150. doi: 10.1016/j.jgar.2022.01.001. Epub 2022 Jan 10.
Using a hybrid long-read sequencing approach, we aimed to fully characterise four extensively-drug resistant (XDR) hypervirulent Klebsiella pneumoniae isolates, one of which represented the first strain isolated in Italy co-expressing NDM-1/5 and OXA-48 carbapenemases.
Whole-genome sequencing was performed using Illumina and Oxford Nanopore Technology platforms. An assembly pipeline was used to recover the structures both of the chromosome and plasmids.
Multilocus sequence typing (MLST) showed that these strains belonged to high-risk sequence types (STs) not commonly circulating in Italy (ST383, ST147 and ST15). The hybrid sequencing approach allowed to characterise three multidrug resistance plasmids, which demonstrated high homology with previously sequenced plasmids, that were simultaneously detected in one ST383 strain carrying, respectively, bla, bla and bla.
This is the first report in Italy of new hypervirulent XDR K. pneumoniae clones characterised by co-production of OXA-48, NDM-1 and NDM-5. The discovery of new high-risk clones harbouring multiple mobile elements is a growing problem that poses a great challenge for public health.
采用混合长读测序方法，我们旨在全面描述四个广泛耐药（XDR）超毒力肺炎克雷伯菌分离株，其中一个代表了在意大利首次分离的同时表达 NDM-1/5 和 OXA-48 碳青霉烯酶的菌株。
使用 Illumina 和 Oxford Nanopore 技术平台进行全基因组测序。采用组装管道来恢复染色体和质粒的结构。
多位点序列分型（MLST）显示这些菌株属于意大利不常见的高风险序列类型（ST）（ST383、ST147 和 ST15）。混合测序方法允许对三个多药耐药质粒进行特征描述，这些质粒与以前测序的质粒具有高度同源性，同时在一个携带 bla、bla 和 bla 的 ST383 菌株中检测到。
这是意大利首次报道的新型超毒力 XDR 肺炎克雷伯菌克隆，其特征是同时产生 OXA-48、NDM-1 和 NDM-5。携带多种移动元件的新高危克隆的发现是一个日益严重的问题，对公共卫生构成了巨大挑战。
