Ma Xiang, Wang Gang, Fan Hao, Li Zengliang, Chen Wangwang, Xiao Jian, Ni Peidong, Liu Kanghui, Shen Kuan, Wang Yuanhang, Xu Zekuan, Yang Li
Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Department of General Surgery, Liyang People's Hospital, Liyang Branch Hospital of Jiangsu Province Hospital, Liyang, Jiangsu, China.
Cell Death Discov. 2022 Jan 11;8(1):20. doi: 10.1038/s41420-021-00809-1.
Gastric cancer (GC) is a global health problem and further studies of its molecular mechanisms are needed to identify effective therapeutic targets. Although some long noncoding RNAs (lncRNAs) have been found to be involved in the progression of GC, the molecular mechanisms of many GC-related lncRNAs remain unclear. In this study, a series of in vivo and in vitro assays were performed to study the relationship between FAM225A and GC, which showed that FAM225A levels were correlated with poor prognosis in GC. Higher FAM225A expression tended to be correlated with a more profound lymphatic metastasis rate, larger tumor size, and more advanced tumor stage. FAM225A also promoted gastric cell proliferation, invasion, and migration. Further mechanistic investigation showed that FAM225A acted as a miR-326 sponge to upregulate its direct target PADI2 in GC. Overall, our findings indicated that FAM225A promoted GC development and progression via a competitive endogenous RNA network of FAM225A/miR-326/PADI2 in GC, providing insight into possible therapeutic targets and prognosis of GC.
胃癌（GC）是一个全球性的健康问题，需要进一步研究其分子机制以确定有效的治疗靶点。尽管已发现一些长链非编码RNA（lncRNA）参与胃癌的进展，但许多与胃癌相关的lncRNA的分子机制仍不清楚。在本研究中，进行了一系列体内和体外试验以研究FAM225A与胃癌的关系，结果表明FAM225A水平与胃癌患者的不良预后相关。较高的FAM225A表达往往与更高的淋巴转移率、更大的肿瘤大小和更晚期的肿瘤分期相关。FAM225A还促进胃细胞增殖、侵袭和迁移。进一步的机制研究表明，FAM225A作为miR-326的海绵，上调其在胃癌中的直接靶点PADI2。总体而言，我们的研究结果表明，FAM225A通过FAM225A/miR-326/PADI2的竞争性内源RNA网络促进胃癌的发展和进展，为胃癌可能的治疗靶点和预后提供了见解。
