高频产生分泌针对细胞抗原的抗体的杂交瘤。

High frequency production of hybridomas secreting antibodies to cell antigens.

作者信息

Cianfriglia M, Nuti M, Turchi V, Barca S, Tombesi M, Morrone S, Cenciarelli C, Natali P G

机构信息

Laboratorio Biologia Cellulare, Istituto Superiore di Sanita, Roma, Italy.

出版信息

Hybridoma. 1987 Dec;6(6):673-7. doi: 10.1089/hyb.1987.6.673.

DOI:10.1089/hyb.1987.6.673

PMID:3501765

Abstract

The efficiency of various immunization protocols for the production of hybridomas secreting immunoglobulins specific to cell antigens was evaluated in 15 different fusion experiments. Some of these experiments were performed with splenic B-lymphocytes from mice at different stages of immunization. This approach allowed a dynamic analysis of immunocompetent splenic B-lymphocyte production during the immunization cycle.

摘要

在15个不同的融合实验中评估了各种免疫方案对于产生分泌针对细胞抗原的特异性免疫球蛋白的杂交瘤的效率。其中一些实验使用了处于不同免疫阶段的小鼠脾脏B淋巴细胞进行。这种方法能够对免疫周期中具有免疫活性的脾脏B淋巴细胞生成进行动态分析。