Raza Shahid, Gautam Hitender, Mohapatra Sarita, Sood Seema, Dhawan Benu, Chaudhry Rama, Kapil Arti, Das Bimal Kumar
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
J Glob Infect Dis. 2021 Nov 9;13(4):177-179. doi: 10.4103/jgid.jgid_404_20. eCollection 2021 Oct-Dec.
Multidrug-resistant is galloping, posing threat to tackle, and leaving us with limited options of treatment.
The purpose of this study is to find the genotypic association in drug-resistant isolated from different sterile body fluids. Matrix-assisted laser desorption/ionization-time of flight confirmed isolates were taken and minimum inhibitory concentration (MIC) was determined by VITEK-2 AST system. The presence of resistance nodulation-division (RND)-efflux pump genes AdeABC-RS was detected by multiplex polymerase chain reaction.
Of the total 40 , 32 (80%) were multidrug resistant though all isolates were susceptible to Tigecycline. Similarly, 26 (81.25%) isolates were positive for RND-efflux pump genes AdeABC-RS.
RND efflux pump AdeABC-RS system plays a significant role in emerging multi drug resistant . Mutation in AdeS gene deciphers the role of regulatory gene. Hence, antimicrobial stewardship should be strictly followed and efflux pump inhibiting substances should be vigorously searched to bring back the era of existing antibiotics.
多重耐药菌正在迅速蔓延，对治疗构成威胁，使我们的治疗选择有限。
本研究的目的是寻找从不同无菌体液中分离出的耐药菌的基因型关联。采用基质辅助激光解吸/电离飞行时间质谱确认分离株，并通过VITEK-2 AST系统测定最低抑菌浓度（MIC）。通过多重聚合酶链反应检测耐药结节-分裂（RND）-外排泵基因AdeABC-RS的存在。
在总共40株菌中，32株（80%）为多重耐药菌，不过所有分离株对替加环素敏感。同样，26株（81.25%）分离株的RND-外排泵基因AdeABC-RS呈阳性。
RND外排泵AdeABC-RS系统在新出现的多重耐药菌中起重要作用。AdeS基因突变解释了调节基因的作用。因此，应严格遵循抗菌药物管理原则，并大力寻找外排泵抑制物质，以恢复现有抗生素的时代。
