Yoshimoto A, Kawamura A, Chinota M
Kotobiken Medical Laboratory, Tokyo, Japan.
Radioisotopes. 1987 Oct;36(10):526-9. doi: 10.3769/radioisotopes.36.10_526.
The radioallergosorbent test (RAST) provides a reliable method for detecting of specific IgE antibodies against a wide variety of antigens. The method using tube added disc for the estimation have been widely used in routine assay. In this study, the method using tray added disc has developed in our laboratory. The results were as follows; (1) The method using tray showed good correlation to the original method. (2) Characteristics of the method are rapidity, simplicity and economics. (3) The reaction well of tray is flat bottomed and is good for disc reaction. (4) Coefficients of variation for intra assay were; (d1): 9.40-9.68%, inter assay were 10.39-12.15% respectively. (5) The seasonal variation of positive allergens and the correlation of d to h, g1 to g3 were estimated. The method using tray seems to be one of the most useful routine methods of RAST assay and checking the seasonal variation of RAST will give good advantages to estimate the regional pollution of pollens allergy.
放射变应原吸附试验（RAST）为检测针对多种抗原的特异性IgE抗体提供了一种可靠的方法。使用试管加盘进行评估的方法已广泛应用于常规检测。在本研究中，我们实验室开发了使用托盘加盘的方法。结果如下：（1）使用托盘的方法与原方法具有良好的相关性。（2）该方法的特点是快速、简便且经济。（3）托盘的反应孔为平底，有利于盘反应。（4）批内变异系数为；（d1）：9.40 - 9.68%，批间变异系数分别为10.39 - 12.15%。（5）估计了阳性变应原的季节变化以及d与h、g1与g3的相关性。使用托盘的方法似乎是RAST检测中最有用的常规方法之一，检测RAST的季节变化将有助于评估花粉过敏的区域污染情况。
