Address Université de Paris, Lab Chim & Biochim Pharmacolog & Toxicol, UMR 8601, F-75006 Paris, France.
Université de Paris, ITODYS, UMR 7086, 75013 Paris, France.
Nanoscale. 2022 Jan 27;14(4):1452-1458. doi: 10.1039/d1nr03148a.
The surface functionalization of silver nanoparticles (NPs) by Raman reporters has stimulated a wide interest in recent years for the design of Surface-Enhanced Raman Spectroscopy (SERS) labels. However, silver NPs are prone to oxidation and aggregation, which strongly limits their applications. The design of stable SERS tags based on Ag NPs still represents a major challenge for Raman bioimaging. We address this issue herein by taking advantage of aryl diazonium salt chemistry to obtain stable Ag NPs functionalized by multifunctional polyaryl layers bearing different Raman reporters (-NO, -CN, -CCH). The resulting SERS-encoded Ag NPs were characterized by UV-vis absorption, transmission electron microscopy (TEM) and SERS. The formation of multilayers at the surface of Ag NPs gives access to new spectrally distinguishable SERS codes thus broadening the library of available Raman tags. Proof-of-concept Raman imaging experiments were performed on cancer cells (HeLa) after NP uptake, highlighting the large potentials of diazonium salt chemistry to design Ag NPs-based SERS labels for Raman bioimaging.
近年来，通过拉曼报告基团对银纳米粒子（NPs）进行表面功能化，激发了人们对表面增强拉曼光谱（SERS）标记物设计的广泛兴趣。然而，银 NPs 容易氧化和聚集，这强烈限制了它们的应用。基于 Ag NPs 的稳定 SERS 标签的设计仍然是 Raman 生物成像的一个主要挑战。我们利用芳基重氮盐化学来获得多功能多芳基层稳定的 Ag NPs 功能化，这些多芳基层带有不同的拉曼报告基团（-NO、-CN、-CCH），从而解决了这个问题。所得的 SERS 编码 Ag NPs 通过紫外-可见吸收、透射电子显微镜（TEM）和 SERS 进行了表征。Ag NPs 表面的多层形成可以获得新的光谱可区分的 SERS 编码，从而扩展了可用的 Raman 标签库。在细胞摄取后，对癌细胞（HeLa）进行了基于 SERS 的 Raman 成像实验，证明了重氮盐化学在设计基于 Ag NPs 的 SERS 标签用于 Raman 生物成像方面具有很大的潜力。
