Lu Zhou, Mondarte Evan A Q, Suthiwanich Kasinan, Hayashi Tomohiro, Masuda Tsukuru, Isu Norifumi, Takai Madoka
Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8656 Tokyo, Japan.
Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8502 Kanagawa, Japan.
ACS Appl Bio Mater. 2020 Feb 17;3(2):1079-1087. doi: 10.1021/acsabm.9b01041. Epub 2020 Jan 28.
Bacterial adhesion on material surfaces is a significant problem in many areas, especially on medical devices. Upon colonizing a surface, bacteria tend to form biofilms and become difficult to eradicate. A multistep process is involved in bacterial biofilm formation, including primary adhesion to material surface and accumulation of bacterial cells. Controlling the primary adhesion of bacteria is an efficient way to manage biofilms. This study focused on the primary adhesion of bacteria on a copolymer thin-film composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), 3-methacryloxypropyl trimethoxysilane (MPTMSi), and 3-(methacryloyloxy) propyl-tris(trimethylsilyloxy) silane (MPTSSi), which has anti-biofouling and thickness and stiffness tunable properties. We modulated the thickness (5-90 nm) and stiffness of the thin-film via changing the polymer concentration in the coating solution (dip coating). All polymer thin-films inhibited Gram-positive and Gram-negative primary adhesions. Interestingly, adhesion was affected by the thickness and/or stiffness of the thin-film. We conclude that the mechanical property of the thin-film is one of the influential factors determining bacterial adhesion. These findings would be of significance in designing antibacterial materials.
细菌在材料表面的黏附是许多领域中的一个重大问题，尤其是在医疗设备上。在定殖于表面后，细菌倾向于形成生物膜，并且变得难以根除。细菌生物膜的形成涉及一个多步骤过程，包括对材料表面的初始黏附以及细菌细胞的聚集。控制细菌的初始黏附是管理生物膜的一种有效方法。本研究聚焦于细菌在由2-甲基丙烯酰氧乙基磷酰胆碱（MPC）、3-甲基丙烯酰氧基丙基三甲氧基硅烷（MPTMSi）和3-（甲基丙烯酰氧基）丙基-三（三甲基硅氧基）硅烷（MPTSSi）组成的共聚物薄膜上的初始黏附，该共聚物薄膜具有抗生物污染以及厚度和刚度可调节的特性。我们通过改变涂布溶液（浸涂）中的聚合物浓度来调节薄膜的厚度（5-90纳米）和刚度。所有聚合物薄膜都抑制革兰氏阳性菌和革兰氏阴性菌的初始黏附。有趣的是，黏附受到薄膜厚度和/或刚度的影响。我们得出结论，薄膜的机械性能是决定细菌黏附的影响因素之一。这些发现对于设计抗菌材料具有重要意义。
