Backlund Coralie M, Hango Christopher R, Minter Lisa M, Tew Gregory N
Department of Polymer Science & Engineering, University of Massachusetts, Amherst, Massachusetts 01003, United States.
Molecular and Cellular Biology Program, University of Massachusetts, Amherst, Massachusetts 01003, United States.
ACS Appl Bio Mater. 2020 Jan 21;3(1):180-185. doi: 10.1021/acsabm.9b00876. Epub 2019 Dec 13.
Intracellular protein delivery using simple noncovalent carriers is an emerging field advancing the study of intracellular pathways and novel therapeutics. Here, we directly compare green fluorescent protein delivery using our recently reported protein transduction domain mimic (PTDM) to delivery with four commercially available amphiphilic macromolecular carriers in five diverse cell types. While most carriers succeeded only in serum-free conditions, the PTDM maintained robust delivery in complete media, even when tasked with antibody delivery into difficult-to-transfect neurons for the first time. The broad effectiveness of this reagent establishes PTDMs as a promising strategy for the delivery of biologics into such sensitive and challenging cell types.
利用简单的非共价载体进行细胞内蛋白质递送是一个新兴领域，正在推动细胞内信号通路研究和新型疗法的发展。在此，我们将我们最近报道的蛋白质转导结构域模拟物（PTDM）介导的绿色荧光蛋白递送与四种市售两亲性大分子载体在五种不同细胞类型中的递送进行了直接比较。虽然大多数载体仅在无血清条件下成功，但PTDM在完全培养基中仍能保持强大的递送能力，即使首次将其用于将抗体递送至难以转染的神经元中也是如此。该试剂的广泛有效性确立了PTDM作为将生物制剂递送至此类敏感且具有挑战性的细胞类型的一种有前景的策略。
