Pan Animesh, Jakaria Md Golam, Meenach Samantha A, Bothun Geoffrey D
ACS Appl Bio Mater. 2020 Jan 21;3(1):273-281. doi: 10.1021/acsabm.9b00797. Epub 2019 Dec 10.
We report a multifunctional nanotherapeutic platform based on liposomes loaded with drug and iron oxide nanoparticles (IONs) coated with a gold nanoshell synthesized using a polyelectrolyte (layersome) soft templating technique. IONs and gold nanoshells were used to provide combined hyperthermia and triggered drug release via radio frequency (RF) or near-infrared (NIR) stimulation. IONs and the anticancer drug doxorubicin (DOX) were coencapsulated inside liposomes composed of zwitterionic phosphatidylcholine, anionic phosphatidylglycerol, and cholesterol lipids. Coating the magneto-liposomes with positively charged poly-l-lysine enriched the interface with gold anions to form a dense gold nanoshell and protected the structure against deformation and DOX cargo release during shell formation. After modification with thiol-terminated polyethylene glycol, intracellular delivery and release of DOX from the nanostructures was examined in A549 human lung cancer cells. The nanostructures retained their DOX cargo and remained in the cytosol after cellular uptake. Only when triggered by RF or NIR stimuli did the nanostructures release DOX, which then entered the cell nucleus. Compared to the single photothermal therapy or radio frequency treatment, the carriers with combined DOX and RF or NIR stimulation displayed higher therapeutic effect on A549 cells.
我们报告了一种基于脂质体的多功能纳米治疗平台，该脂质体负载有药物以及用聚电解质（层状囊泡）软模板技术合成的包覆金纳米壳的氧化铁纳米颗粒（IONs）。IONs和金纳米壳用于通过射频（RF）或近红外（NIR）刺激提供联合热疗和触发药物释放。IONs和抗癌药物阿霉素（DOX）共包封在由两性离子磷脂酰胆碱、阴离子磷脂酰甘油和胆固醇脂质组成的脂质体内。用带正电荷的聚-L-赖氨酸包覆磁脂质体，使界面富含金阴离子，形成致密的金纳米壳，并在壳形成过程中保护结构不发生变形和DOX货物释放。用硫醇末端的聚乙二醇修饰后，在A549人肺癌细胞中检测了DOX从纳米结构的细胞内递送和释放。纳米结构保留了其DOX货物，并在细胞摄取后留在细胞质中。只有当受到RF或NIR刺激时，纳米结构才会释放DOX，然后DOX进入细胞核。与单一光热疗法或射频治疗相比，具有DOX与RF或NIR联合刺激的载体对A549细胞显示出更高的治疗效果。
