Michel Sarah E S, Rogers Sarah E, Briscoe Wuge H, Galan M Carmen
School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, U.K.
ISIS Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Didcot, OX11 0QX, U.K.
ACS Appl Bio Mater. 2020 Nov 16;3(11):8075-8083. doi: 10.1021/acsabm.0c01171. Epub 2020 Oct 27.
Access to biocompatible hydrogels with tunable properties is of great interest in biomedical applications. Here we report the synthesis and characterization of a series of photo-cross-linked chitosan hydrogels from norbornene-functionalized chitosan (CS-nb) and various thiolated cross-linkers. The resulting materials were characterized by NMR, swelling ratio, rheology, SEM, and small angle neutron scattering (SANS) measurements. The hydrogels exhibited pH- and salt-dependent swelling, while the macro- and microscale properties could be modulated by the choice and degree of cross-linker or the polymer concentration. The materials could be molded in situ and loaded with small molecules that can be released overtime. Moreover, the incorporation of collagen in the hydrogels drastically improved cell adhesion, with excellent viabilities of human dermofibroblast cells on the hydrogels observed for up to 6 days, highlighting the potential use of these materials in the biomedical area.
获得具有可调节性质的生物相容性水凝胶在生物医学应用中具有极大的吸引力。在此，我们报告了一系列由降冰片烯功能化壳聚糖（CS-nb）和各种硫醇化交联剂合成的光交联壳聚糖水凝胶及其表征。通过核磁共振（NMR）、溶胀率、流变学、扫描电子显微镜（SEM）和小角中子散射（SANS）测量对所得材料进行了表征。这些水凝胶表现出pH和盐依赖性溶胀，而宏观和微观性质可通过交联剂的选择和程度或聚合物浓度进行调节。这些材料可以原位成型并负载小分子，小分子可随时间释放。此外，在水凝胶中加入胶原蛋白极大地改善了细胞黏附，在水凝胶上观察到人类皮肤成纤维细胞具有长达6天的优异活力，突出了这些材料在生物医学领域的潜在用途。
