Eliyahu Shaked, Galitsky Alexandra, Ritov Esther, Bianco-Peled Havazelet
The Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
Department of Chemical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
Polymers (Basel). 2021 Jan 14;13(2):266. doi: 10.3390/polym13020266.
We developed and characterized a new hydrogel system based on the physical and chemical interactions of pectin partially modified with thiol groups and chitosan modified with acrylate end groups. Gelation occurred at high pectin thiol ratios, indicating that a low acrylated chitosan concentration in the hydrogel had a profound effect on the cross-linking. Turbidity, Fourier transform infrared spectroscopy, and free thiol determination analyses were performed to determine the relationships of the different bonds inside the gel. At low pH values below the pKa of chitosan, more electrostatic interactions were formed between opposite charges, but at high pH values, the Michael-type addition reaction between acrylate and thiol took place, creating harder hydrogels. Swelling experiments and Young's modulus measurements were performed to study the structure and properties of the resultant hydrogels. The nanostructure was examined using small-angle X-ray scattering. The texture profile analysis showed a unique property of hydrogel adhesiveness. By implementing changes in the preparation procedure, we controlled the hydrogel properties. This hybrid hydrogel system can be a good candidate for a wide range of biomedical applications, such as a mucosal biomimetic surface for mucoadhesive testing.
我们基于硫醇基团部分修饰的果胶与丙烯酸酯端基修饰的壳聚糖之间的物理和化学相互作用，开发并表征了一种新型水凝胶体系。在高果胶硫醇比例下发生凝胶化，这表明水凝胶中低浓度的丙烯酸化壳聚糖对交联有深远影响。进行了浊度、傅里叶变换红外光谱和游离硫醇测定分析，以确定凝胶内部不同键之间的关系。在低于壳聚糖pKa的低pH值下，相反电荷之间形成更多静电相互作用，但在高pH值下，丙烯酸酯和硫醇之间发生迈克尔型加成反应，形成更硬的水凝胶。进行了溶胀实验和杨氏模量测量，以研究所得水凝胶的结构和性能。使用小角X射线散射检查纳米结构。质地剖面分析显示了水凝胶粘附性的独特性质。通过改变制备程序，我们控制了水凝胶的性能。这种混合水凝胶体系可成为广泛生物医学应用的良好候选者，例如用于粘膜粘附测试的粘膜仿生表面。
