He Qiyi, Yang Huiyi, Pan Junkang, Cui Xiping, Shen Ding, Eremin Sergei A, Fang Yanxiong, Zhao Suqing
Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, People's Republic of China.
Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, People's Republic of China.
ACS Appl Bio Mater. 2020 Dec 21;3(12):8849-8856. doi: 10.1021/acsabm.0c01169. Epub 2020 Nov 11.
Lateral flow immunoassay (LFIA) is one of the most widely used tools for analysis and field measurement and has the advantages of high efficiency, simple operation, portability, and low cost. Therefore, in this study, we designed a proof-of-concept of LFIA based on rhodium nanoparticles and investigated its improvement by further introducing the tetramethyl benzidine and HO mixture as the substrate to trigger the color reaction. The proposed methods were qualitative research by the naked eye and quantitative measurement by a smartphone and software. Under the optimal condition, the detection of ferritin was successfully established with the limit of detection of 0.3 ng/mL. The lowest visually detectable amount was 0.05 ng/mL. To verify the performance of the RhNPs-LFIA, three spiked serum samples were tested, and the recovery rate increased from 88.9 to 129.9%, revealing that the proposed methods were applicable and practically reliable for testing serum samples. The developed RhNP-based LFIA is highly sensitive and convenient, which provides a promising technology for accurate, rapid, high sensitivity, and high screening detection of ferritin in clinical diagnosis.
侧向流动免疫分析（LFIA）是分析和现场测量中使用最广泛的工具之一，具有高效、操作简单、便携和低成本等优点。因此，在本研究中，我们设计了一种基于铑纳米颗粒的LFIA概念验证，并通过进一步引入四甲基联苯胺和HO混合物作为底物来触发颜色反应，研究了其改进效果。所提出的方法通过肉眼进行定性研究，并通过智能手机和软件进行定量测量。在最佳条件下，成功建立了铁蛋白检测方法，检测限为0.3 ng/mL。最低可视检测量为0.05 ng/mL。为验证RhNPs-LFIA的性能，对三个加标血清样本进行了测试，回收率从88.9%提高到129.9%，表明所提出的方法适用于血清样本检测且实际可靠。所开发的基于RhNP的LFIA高度灵敏且方便，为临床诊断中铁蛋白的准确、快速、高灵敏度和高筛查检测提供了一种有前景的技术。
