Department of Medical Biochemistry, 68843Faculty of Medicine , Minia University, Egypt.
Medical Bichemistry, 63527Kaser AlAiny, Cairo, Egypt.
Lupus. 2022 Jan;31(1):89-96. doi: 10.1177/09612033211067166. Epub 2022 Jan 12.
The skin and the kidney are commonly affected in systemic lupus erythematosus (SLE) with similar molecular mechanisms. Although clinical indicators of renal injury in SLE are fairly uncontroversial, few biomarkers are reliable. The role of micro-RNAs (mi-RNAs) in lupus nephritis (LN) pathogenesis has been investigated to help in early diagnosis.
The aim of work is to evaluate miRNA132 and SOX2 expressions in SLE Egyptian patients; with and without nephritis, and the relation between miRNA132 and its long non-coding gene SOX2 in both patients groups.
This is a case-control study involving 100 SLE patients with and without LN (LN and non-LN groups), and 50 age-and sex-matched healthy controls. The study was carried out to detect miRNA132 and SOX2 expression by quantitative Real-Time Polymerase chain reaction methods. The SLE disease activity index (SLEDAI) was assessed.
SLEDAI increased in LN compared to non-LN. Micro-RNA132 expression was significantly increased in patient groups compared to controls (<0.01) and increased in LN more than non-LN group (). SOX2 significantly decreased in patient groups compared to controls (<0.001), and was more in LN compared to non-LN group (<0.001). There was a negative correlation between miRNA132 and SOX2 expression in both patient groups ().
miRNA132 and SOX2 may play a role in SLE activity and help in the early non-invasive diagnosis of LN.
系统性红斑狼疮（SLE）中皮肤和肾脏常受影响，具有相似的分子机制。尽管 SLE 肾损伤的临床指标相当无争议，但很少有生物标志物是可靠的。微小 RNA（miRNA）在狼疮肾炎（LN）发病机制中的作用已被研究，以帮助早期诊断。
本研究旨在评估埃及 SLE 患者皮肤和肾脏组织中 miRNA132 和 SOX2 的表达情况，及其与 LN 的关系。
这是一项病例对照研究，共纳入 100 例 SLE 患者，包括 LN 组和非 LN 组，以及 50 名年龄和性别匹配的健康对照者。采用定量实时聚合酶链反应方法检测 miRNA132 和 SOX2 的表达。采用系统性红斑狼疮疾病活动指数（SLEDAI）进行评估。
与非 LN 组相比，LN 组的 SLEDAI 升高。与对照组相比，患者组的 miRNA132 表达显著升高（<0.01），且 LN 组的表达高于非 LN 组（）。与对照组相比，患者组的 SOX2 表达显著降低（<0.001），且 LN 组的表达低于非 LN 组（<0.001）。在两组患者中，miRNA132 与 SOX2 表达呈负相关（）。
miRNA132 和 SOX2 可能在 SLE 活动中发挥作用，并有助于 LN 的早期非侵入性诊断。
