Iba K, Morohashi K, Miyata T, Takamiya K
Department of Biology, Faculty of Science, Kyushu University, Fukuoka.
J Biochem. 1987 Dec;102(6):1511-8. doi: 10.1093/oxfordjournals.jbchem.a122198.
The structural gene coding for cytochrome b-562 isolated from the cytochrome b-c1 complex of Rhodobacter (Rhodopseudomonas) sphaeroides has been cloned. Its nucleotide sequence has been determined and the amino acid sequence was deduced therefrom. It consists of 157 amino acids (Mr 17,237) and contains four hydrophobic segments. The first 30 residues in the predicted amino acid sequence are the same as those determined for the NH2-terminal portion of purified cytochrome b-562. The amino acid composition is in accord with that determined for the pure protein. From the hydropathy profile and molar ratio of protoheme to cytochrome b-562, it is suggested that the structural and functional unit of the cytochrome is a two-heme cross-linked homodimer.
已克隆出从球形红杆菌（红假单胞菌）细胞色素b-c1复合体中分离出的编码细胞色素b-562的结构基因。已确定其核苷酸序列，并由此推导了氨基酸序列。它由157个氨基酸组成（分子量17,237），包含四个疏水片段。预测的氨基酸序列中的前30个残基与纯化的细胞色素b-562的NH2末端部分所确定的残基相同。氨基酸组成与纯蛋白所确定的一致。从亲水性图谱和原血红素与细胞色素b-562的摩尔比来看，表明细胞色素的结构和功能单位是双血红素交联同二聚体。
