Li Liangliang, Li Shuo, Qin Shidong, Gao Yu, Wang Chao, Du Jinghang, Zhang Nannan, Chen Yanbo, Han Zhen, Yu Yue, Wang Fan, Zhao Yashuang
Department of Epidemiology, School of Public Health, Harbin Medical University, Harbin, China.
The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Front Nutr. 2021 Dec 8;8:747964. doi: 10.3389/fnut.2021.747964. eCollection 2021.
DNA methylation is influenced by environmental factors and contributes to adverse modification of cancer risk and clinicopathological features. A case-control study (402 newly diagnosed cases, 470 controls) was conducted to evaluate the effect of environmental factors and methylation in peripheral blood leukocyte (PBL) DNA on the risk of breast cancer. A case-only study (373 cases) was designed to evaluate the effects of environmental factors on methylation in tumor tissue and the relationship of methylation with clinicopathological features. We found a significant association between hypermethylation of and the risk of breast cancer (OR = 1.914, 95%CI = 1.357-2.777). hypermethylation in PBL DNA combined with low intake of vegetable, garlic, soybean, poultry, and milk; high pork intake; less regular sports and a high psychological stress index significantly increased the risk of breast cancer. Soybean intake (OR = 0.425, 95%CI: 0.231-0.781) and regular sports (OR = 0.624, 95%CI: 0.399-0.976) were associated with hypermethylation in tumor DNA. hypermethylation in tumor tissue was correlated with estrogen receptor (ER) (OR = 1.945, 95%CI: 1.262-2.996) and progesterone receptor (PR) (OR = 1.611, 95%CI: 1.069-2.427) negative status; in addition, hypermethylation in PBL DNA was associated with human epidermal growth factor receptor 2 (HER-2) negative status (OR = 3.673, 95%CI: 1.411-9.564). A healthy diet, psychosocial adaptability, and regular sports are very beneficial for breast cancer prevention and progress, especially for hypermethylation carriers. Personalized treatment considering the correlation between hypermethylation and ER and PR status may provide a novel benefit for breast cancer patients.
DNA甲基化受环境因素影响，并导致癌症风险和临床病理特征的不良改变。开展了一项病例对照研究（402例新诊断病例，470例对照），以评估环境因素和外周血白细胞（PBL）DNA甲基化对乳腺癌风险的影响。设计了一项病例单组研究（373例病例），以评估环境因素对肿瘤组织甲基化的影响以及甲基化与临床病理特征的关系。我们发现[基因名称]高甲基化与乳腺癌风险之间存在显著关联（OR = 1.914，95%CI = 1.357 - 2.777）。PBL DNA中的[基因名称]高甲基化与蔬菜、大蒜、大豆、家禽和牛奶摄入量低；猪肉摄入量高；体育锻炼不规律以及心理压力指数高显著增加乳腺癌风险。大豆摄入量（OR = 0.425，95%CI：0.231 - 0.781）和规律体育锻炼（OR = 0.624，95%CI：0.399 - 0.976）与肿瘤DNA中的[基因名称]高甲基化有关。肿瘤组织中的[基因名称]高甲基化与雌激素受体（ER）（OR = 1.945，95%CI：1.262 - 2.996）和孕激素受体（PR）（OR = 1.611，95%CI：1.069 - 2.427）阴性状态相关；此外，PBL DNA中的[基因名称]高甲基化与人类表皮生长因子受体2（HER - 2）阴性状态相关（OR = 3.673，95%CI：1.411 - 9.564）。健康的饮食、心理社会适应能力和规律的体育锻炼对乳腺癌的预防和进展非常有益，尤其是对于[基因名称]高甲基化携带者。考虑到[基因名称]高甲基化与ER和PR状态之间的相关性进行个性化治疗可能为乳腺癌患者带来新的益处。
