酵母tRNA3Arg摆动位置上5-羧甲基尿苷甲酯的配对特性
Pairing properties of the methylester of 5-carboxymethyl uridine in the wobble position of yeast tRNA3Arg.
作者信息
Weissenbach J, Dirheimer G
出版信息
Biochim Biophys Acta. 1978 May 23;518(3):530-4. doi: 10.1016/0005-2787(78)90171-5.
PMID:350282
Abstract
At optimum magnesium concentration (10 mM) both yeast tRNA1Arg and tRNA3Arg are able to bind to poly (A,G) and A-G-A in presence of Escherichia coli robisomes. With A-G-G only tRNA1Arg ginds, wherea tRNA3Arg (anticodon mcm5 U-C-U) is not bound. This result means that the methylcarboxymethyl substituant in position 5 of U prevents its wobble with G.
摘要
在最佳镁离子浓度（10 mM）下，酵母tRNA1Arg和tRNA3Arg在大肠杆菌核糖体存在的情况下都能够与聚（A，G）和A - G - A结合。对于A - G - G，只有tRNA1Arg能够结合，而tRNA3Arg（反密码子mcm5U - C - U）不结合。这一结果意味着U第5位的甲基羧甲基取代基阻止了它与G的摆动配对。
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