长非编码 RNA RP11-342L8.2 来源于 RNA 测序，并经 RT-qPCR 验证，在银屑病患者中上调，并与疾病严重程度相关。
Long non-coding RNA RP11-342L8.2, derived from RNA sequencing and validated via RT-qPCR, is upregulated and correlates with disease severity in psoriasis patients.
机构信息
Department of Dermatology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
出版信息
Ir J Med Sci. 2022 Dec;191(6):2643-2649. doi: 10.1007/s11845-021-02882-y. Epub 2022 Jan 14.
OBJECTIVE
This study aimed to explore the dysregulated long non-coding RNA (lncRNA) expression profile in psoriatic tissue vs. normal skin tissue via RNA sequencing (RNA-seq), then further sort candidate lncRNAs to be validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR), in order to investigate the comprehensive linkage of lncRNA with psoriasis.
METHODS
Twenty-five psoriasis patients were consecutively enrolled, with their psoriatic and surrounding normal skin tissues obtained. Ten pairs of psoriatic and normal tissues were proposed to RNA-seq. Then, top 6 differentially expressed lncRNAs (DElncRNA) were sorted as candidate lncRNAs for validation by RT-qPCR in 25 pairs of samples.
RESULTS
Principal component analysis (PCA) exhibited that lncRNA profile clearly distinguished psoriatic tissue from normal tissue, so did heatmap. Volcano plot disclosed 412 upregulated and 625 downregulated DElncRNAs in psoriatic tissue vs. normal tissue. Gene Ontology (GO) and Kyoko Encyclopedia of Genes and Genomes (KEGG) enrichment analyses exhibited that these DElncRNAs were mainly enriched in immune, inflammation, or proliferation-related biological processes and pathways such as neutrophil degranulation, regulation of immune response, positive regulation of cell proliferation, and MAPK signaling pathway. By RT-qPCR validation, lncRNAs RP11-22A3.2, RP11-342L8.2, and CTD-2006H14.2 were increased (all P < 0.001), while lncRNAs AP000442.4, CCDC144NL-AS1, and MIR663AHG were decreased (all P < 0.01) in psoriatic tissue vs. normal tissue. Interestingly, psoriatic lncRNA RP11-342L8.2 was also observed to positively correlated with psoriasis area and severity index (PASI) (r = 0.405, P = 0.045).
CONCLUSION
Our present study exhibits some evidence for the landscape of lncRNAs implicated in psoriasis.
目的
通过 RNA 测序（RNA-seq）探索银屑病组织与正常皮肤组织之间失调的长非编码 RNA（lncRNA）表达谱，然后进一步筛选候选 lncRNA 并通过逆转录定量聚合酶链反应（RT-qPCR）进行验证，以研究 lncRNA 与银屑病的综合关联。
方法
连续纳入 25 例银屑病患者，获取其银屑病和周围正常皮肤组织。提出 10 对银屑病和正常组织进行 RNA-seq。然后，在 25 对样本中，通过 RT-qPCR 对排名前 6 的差异表达 lncRNA（DElncRNA）进行候选 lncRNA 验证。
结果
主成分分析（PCA）表明 lncRNA 谱能清楚地区分银屑病组织和正常组织，热图也是如此。火山图显示银屑病组织中 412 个上调和 625 个下调的 DElncRNA。基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，这些 DElncRNA 主要富集在免疫、炎症或增殖相关的生物学过程和途径中，如嗜中性粒细胞脱颗粒、免疫反应调节、细胞增殖的正调节和 MAPK 信号通路。通过 RT-qPCR 验证，lncRNA RP11-22A3.2、RP11-342L8.2 和 CTD-2006H14.2 表达增加（均 P<0.001），而 lncRNA AP000442.4、CCDC144NL-AS1 和 MIR663AHG 在银屑病组织中表达降低（均 P<0.01）。有趣的是，银屑病 lncRNA RP11-342L8.2 也与银屑病面积和严重程度指数（PASI）呈正相关（r=0.405，P=0.045）。
结论
本研究为银屑病相关 lncRNA 的研究提供了一些证据。
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