Department of Conservative Dentistry, Medical University of Warsaw, 02-091 Warszawa, Poland.
Department of Methodology, Medical University of Warsaw, Żwirki i Wigury 61, 02-097 Warszawa, Poland.
Biomolecules. 2022 Jan 15;12(1):138. doi: 10.3390/biom12010138.
Lipopolysaccharide (LPS) is widely used for induction of inflammation in various human tissues, including dental pulp. The purpose of this study was to summarize current medical literature focusing on (1) cell types used by researchers to simulate dental pulp inflammation, (2) LPS variants utilized in experimental settings and how these choices affect the findings. Our study was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). We searched for studies reporting outcomes of lipopolysaccharide application on dental pulp cells in vitro using electronic databases: MEDLINE, Web of Science and Scopus. Having gathered data from 115 papers, we aimed to present all known effects LPS has on different cell types present in dental pulp. We focused on specific receptors and particles that are involved in molecular pathways. Our review provides an essential foundation for further research using in vitro models of pulpitis.
脂多糖（LPS）广泛用于诱导包括牙髓在内的各种人体组织的炎症。本研究的目的是总结目前医学文献中关于（1）研究人员用于模拟牙髓炎症的细胞类型，（2）实验中使用的 LPS 变体以及这些选择如何影响研究结果。我们的研究符合系统评价和荟萃分析的首选报告项目（PRISMA）。我们使用电子数据库 MEDLINE、Web of Science 和 Scopus 搜索报告体外应用脂多糖对牙髓细胞影响的研究。从 115 篇论文中收集数据后，我们旨在展示 LPS 对牙髓中不同细胞类型的所有已知影响。我们专注于参与分子途径的特定受体和颗粒。本综述为使用牙髓炎的体外模型进行进一步研究提供了重要基础。
