Baci Gabriela-Maria, Cucu Alexandra-Antonia, Giurgiu Alexandru-Ioan, Muscă Adriana-Sebastiana, Bagameri Lilla, Moise Adela Ramona, Bobiș Otilia, Rațiu Attila Cristian, Dezmirean Daniel Severus
Faculty of Animal Science and Biotechnology, University of Animal Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania.
Faculty of Biology, University of Bucharest, 050095 Bucharest, Romania.
Insects. 2021 Dec 27;13(1):28. doi: 10.3390/insects13010028.
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) represents a powerful genome editing technology that revolutionized in a short period of time numerous natural sciences branches. Therefore, extraordinary progress was made in various fields, such as entomology or biotechnology. is one of the most important insects, not only for the sericulture industry, but for numerous scientific areas. The silkworms play a key role as a model organism, but also as a bioreactor for the recombinant protein production. Nowadays, the CRISPR-Cas genome editing system is frequently used in order to perform gene analyses, to increase the resistance against certain pathogens or as an imaging tool in . Here, we provide an overview of various studies that made use of CRISPR-Cas for genome editing, with a focus on emphasizing the high applicability of this system in entomology and biological sciences.
CRISPR（成簇规律间隔短回文重复序列）-Cas（CRISPR相关蛋白）代表了一种强大的基因组编辑技术，它在短时间内彻底改变了众多自然科学分支。因此，在各个领域都取得了非凡的进展，如昆虫学或生物技术领域。家蚕是最重要的昆虫之一，不仅对养蚕业而言，对众多科学领域也是如此。家蚕作为模式生物发挥着关键作用，同时也作为重组蛋白生产的生物反应器。如今，CRISPR-Cas基因组编辑系统经常被用于进行基因分析、增强对某些病原体的抗性或作为家蚕中的成像工具。在此，我们概述了利用CRISPR-Cas进行家蚕基因组编辑的各种研究，重点强调该系统在昆虫学和生物科学中的高度适用性。
