Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, PR China; Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, PR China; Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
J Insect Physiol. 2021 Nov-Dec;135:104325. doi: 10.1016/j.jinsphys.2021.104325. Epub 2021 Oct 30.
Clustered regularly spaced short palindrome repeats (CRISPR) structure family forms the acquired immune system in bacteria and archaea. Recent advances in CRISPR/Cas genome editing as derived from prokaryotes, confirmed the characteristics of robustness, high target specificity and programmability, and also revolutionized the insect sciences field. The successful application of CRISPR in a wide variety of lepidopteran insects, with a high genetic diversity, provided opportunities to explore gene functions, insect modification and pest control. In this review, we present a detailed overview on the recent progress of CRISPR in lepidopteran insects, and described the basic principles of the system and its application. Major interest is on wing development, pigmentation, mating, reproduction, sex determination, metamorphosis, resistance and silkworm breeding innovation. Finally, we outlined the limitations of CRISPR/Cas system and discussed its application prospects in lepidopteran insects.
成簇规律间隔短回文重复（CRISPR）结构家族在细菌和古菌中形成获得性免疫系统。最近在源自原核生物的 CRISPR/Cas 基因组编辑方面的进展，证实了其具有强大、高靶标特异性和可编程性的特点，也彻底改变了昆虫科学领域。CRISPR 在具有高度遗传多样性的各种鳞翅目昆虫中的成功应用，为探索基因功能、昆虫修饰和害虫防治提供了机会。在这篇综述中，我们详细介绍了 CRISPR 在鳞翅目昆虫中的最新进展，并描述了该系统的基本原理及其应用。主要关注点在于翅膀发育、色素沉着、交配、繁殖、性别决定、变态、抗性和家蚕养殖创新。最后，我们概述了 CRISPR/Cas 系统的局限性，并讨论了其在鳞翅目昆虫中的应用前景。
