Wu Zhongdong, Xiao Mingshu, Lai Wei, Sun Yueyang, Li Li, Hu Zongqian, Pei Hao
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
ACS Appl Bio Mater. 2022 May 16;5(5):1901-1915. doi: 10.1021/acsabm.1c01126. Epub 2022 Jan 21.
The cell membrane is a biological interface regulating the communications between cells and their environment. The ability to functionalize the cell membrane with molecules or nanomaterials allows us to manipulate cellular behaviors and to expand cellular functions. Due to their unique merits of synthetic accessibility, flexible design, and precise programmability, nucleic acids provide an emerging and promising molecular toolkit for cell surface engineering. In this review, the recent progress in nucleic acid-based cell surface engineering are summarized. We first introduce approaches to nucleic acid-based cell surface engineering, including monovalent and polyvalent surface engineering strategies. Then, the biological applications of nucleic acid-based cell surface engineering in biosensing of extracellular microenvironment, programming cell-cell interactions, and mimicking cellular behaviors are reviewed. Finally, we analyze the current challenges existing in this area and discuss the prospects for the future development.
细胞膜是调节细胞与其环境之间通讯的生物界面。用分子或纳米材料对细胞膜进行功能化的能力使我们能够操纵细胞行为并扩展细胞功能。由于其在合成可及性、灵活设计和精确可编程性方面的独特优点，核酸为细胞表面工程提供了一种新兴且有前景的分子工具包。在本综述中，总结了基于核酸的细胞表面工程的最新进展。我们首先介绍基于核酸的细胞表面工程方法，包括单价和多价表面工程策略。然后，综述了基于核酸的细胞表面工程在细胞外微环境生物传感、编程细胞间相互作用和模拟细胞行为方面的生物学应用。最后，我们分析了该领域目前存在的挑战，并讨论了未来发展的前景。
